Genetic Analysis of Thai Centella asiatica Germplasm for Morphological, Biomass, and Centelloside Traits

Abstract

Centella asiatica (L.) Urban or Asiatic pennywort is an important medicinal plant used in traditional medicine, cosmetic and pharmaceutical industries; it is also an indigenous vegetable in Asian countries. A total of 169 Centella accessions were collected from all regions of Thailand and assessed for 29 traits, including morphological traits, plant biomass, and centelloside contents. Experiments were conducted in two growing systems, soil and hydroponics, with two harvests every month. The centelloside concentrations were determined by high-performance thin-layer chromatography (HPTLC) procedure. Analysis of variance (ANOVA), correlation matrix, genetic parameters, principal component analysis (PCA), and hierarchical clustering were determined both across all environments and for each growing system separately. The ANOVA revealed significant differences in genotypes and growing systems, along with their interactions. Hydroponic culture produced three- to four-fold higher biomass than soil system while triterpenoid sapogenin concentrations were notably greater in hydroponics. Biomass-related traits showed strong positive correlations with centelloside yields, and genetic analyses revealed moderate to high heritability for these characteristics. PCA and cluster analyses classified accessions into four distinct groups, identifying elite genotypes with both high biomass and centelloside yield. These findings provide a solid foundation for targeted selection in Centella breeding programs. © 2025 by the authors.