National Institutes of Natural Sciences (NINS) - Japan; National Institute for Basic Biology (NIBB); National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Russian Academy of Sciences; Timiryazev Institute of Plant Physiology; Ehime University; Kazusa DNA Research Institute; Lomonosov Moscow State University; Graduate University for Advanced Studies - Japan
Type
Article
Source Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Year
2004
Volume
279
Issue
51
Page
53078-53086
Open Access
Green Published, hybrid
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI
10.1074/jbc.M410162200
Format
PDF
Abstract
Microorganisms respond to hyperosmotic stress via changes in the levels of expression of large numbers of genes. Such responses are essential for acclimation to a new osmotic environment. To identify factors involved in the perception and transduction of signals caused by hyperosmotic stress, we examined the response of Synechocystis sp. PCC 6803, which has proven to be a particularly useful microorganism in similar analyses. We screened knockout libraries of histidine kinases (Hiks) and response regulators (Rres) in Synechocystis by DNA microarray and slot-blot hybridization analyses, and we identified several two-component systems, which we designated Hik-Rre systems, namely, Hik33-Rre31, Hik34-Rre1, and Hik10-Rre3, as well as Hik16-Hik41-Rre17, as the transducers of hyperosmotic stress. We also identified Hik2-Rre1 as a putative additional two-component system. Each individual two-component system regulated the transcription of a specific group of genes that were responsive to hyperosmotic stress.
