• Transcriptomic screening of novel targets of sericin in human hepatocellular carcinoma cells

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Metadata

Document Title

Transcriptomic screening of novel targets of sericin in human hepatocellular carcinoma cells

Author

Jantaravinid J. Tirawanchai N. Ampawong S. Kengkoom K. Somkasetrin A. Nakhonsri V. Aramwit P.

Affiliations

Center of Excellence in Bioactive Resources for Innovative Clinical Applications Department of Pharmacy Practice Faculty of Pharmaceutical Sciences Chulalongkorn University 254 Phayathai Road Pathumwan Bangkok 10330 Thailand; Department of Biochemistry Faculty of Medicine Siriraj Hospital Mahidol University 2 Wanglang Road Bangkoknoi Bangkok 10700 Thailand; Department of Tropical Pathology Faculty of Tropical Medicine Mahidol University 420/6 Ratchawithi Road Ratchathewi Bangkok 10400 Thailand; Research and Academic Support Office National Laboratory Animal Center Mahidol University 999 Salaya Puttamonthon Nakorn Pathom 73170 Thailand; National Biobank of Thailand (NBT) National Science and Technology Development Agency (NSTDA) 144 Innovation Cluster 2 Building (INC) Tower A Thailand Science Park Khlong Nueng Khlong Luang District Pathum Thani 12120 Thailand; The Academy of Science The Royal Society of Thailand Dusit Bangkok 10330 Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2024

Volume

14

Issue

1

Open Access

All Open Access Gold

Publisher

Nature Research

DOI

10.1038/s41598-024-56179-y

Abstract

Sericin a natural protein derived from Bombyx mori is known to ameliorate liver tissue damage; however its molecular mechanism remains unclear. Herein we aimed to identify the possible novel targets of sericin in hepatocytes and related cellular pathways. RNA sequencing analysis indicated that a low dose of sericin resulted in 18 differentially expressed genes (DEGs) being upregulated and 68 DEGs being downregulated while 61 DEGs were upregulated and 265 DEGs were downregulated in response to a high dose of sericin (FDR ? 0.05 fold change > 1.50). Functional analysis revealed that a low dose of sericin regulated pathways associated with the complement and coagulation cascade metallothionine and histone demethylate (HDMs) whereas a high dose of sericin was associated with pathways involved in lipid metabolism mitogen-activated protein kinase�(MAPK) signaling and autophagy. The gene network analysis highlighted twelve genes A2M SERPINA5 MT2A MT1G MT1E ARID5B POU2F1 APOB TRAF6 HSPA8 FGFR1 and OGT as novel targets of sericin. Network analysis of transcription factor activity revealed that sericin affects NFE2L2 TFAP2C STAT1 GATA3 CREB1 and CEBPA. Additionally the protective effects of sericin depended on the counterregulation of APOB POU2F1 OGT TRAF6 and HSPA5. These findings suggest that sericin exerts hepatoprotective effects through diverse pathways at different doses providing novel potential targets for the treatment of liver diseases. ? The Author(s) 2024.

License

CC BY

Rights

Authors

Publication Source

WOS

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