Abstract
An aerobic, spore-forming, actinomycete, designated strain CHM3-46(T), was isolated from soil in a hot spring pond located in Chiangmai province, Thailand. The strain exhibited taxonomic characteristics consistent with the genus Thermocatellispora. Strain CHM3-46(T) produced short, straight chains of warty spores on aerial mycelia. The presence of meso-diaminopimelic acid was observed in the cell-wall peptidoglycan. The whole-cell reducing sugars were glucose, mannose, galacose and ribose. The phospholipids comprised phosphatidylmethylethanolamine, hydroxyphosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, four phosphoglycolipids and three unidentified phospholipids. The predominant menaquinones were MK-9(H-4), MK-9(H-6) and MK-9 (H-8). 10-methyl C-17:0, C-16:0 , C-17:0 and iso-C-16:0 were identified as the main cellular fatty acids. The G+C content of the genomic DNA was 73.2 mol%. Analysis of the 16S rRNA gene sequence showed that strain CHM3-46(T) belonged to the genus Thermocatellispora, exhibiting the highest similarity to Thermocatellispora tengchongensis YIM 77521(T )(98.5 %). Furthermore, a low DNA relatedness value (23.4 %+/- 0.8) and several physiological and biochemical characteristic differences were detected between strain CHM3-46(T) and its closest relative. Based on the taxonomic data, strain CHM3-46(T) could be readily distinguished from its closest phylogenetic relative and represents a novel species, for which the name Thermocatellispora soli sp. nov. is proposed. The type strain is CHM3-46(T )(=TBRC 7649(T)=NBRC 113148(T)).
