• The effect of printing parameters on the properties of 17–4 PH stainless steel fabricated by material extrusion additive manufacturing

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06/12/2024 by   

Metadata

Document Title

The effect of printing parameters on the properties of 17–4 PH stainless steel fabricated by material extrusion additive manufacturing

Author

Seensattayawong P., Suwanpreecha C., Boonlert N., Songkuea S., Manonukul A.

Affiliations

Integrative Biomedical Research Unit (IBRU), Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand; Department of Cardio-Thoracic Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand; Biomedical Engineering and Innovation Research Centre, Chiang Mai University, Mueang, Chiang Mai, 50200, Thailand; Biomedical Engineering Institute, Chiang Mai University, Mueang, Chiang Mai, 50200, Thailand; Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand; National Centre for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Science Laboratory Center, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2024

Volume

14

Issue

1

Open Access

All Open Access, Gold

Publisher

Nature Research

DOI

10.1038/s41598-024-64717-x

Abstract

Cigarette smoke (CS) is one of the leading causes of pulmonary diseases and can induce lung secretome alteration. CS exposure-induced damages to human pulmonary epithelial cells and microvascular endothelial cells have been extensively demonstrated; however, the effects of the secretome of lung epithelial cells exposed to CS extracts (CSE) on lung microvascular endothelial cells are not fully understood. In this study, we aimed to determine the effects of the secretome of lung epithelial cells exposed to CSE on lung microvascular endothelial cells. Human lung epithelial cells, A549, were exposed to CSE, and the secretome was collected. Human lung microvascular endothelial cells, HULEC-5a, were used to evaluate the effect of the secretome of A549 exposed to CSE. Secretome profile, endothelial cell death, inflammation, and permeability markers were determined. CSE altered the secretome expression of A549 cells, and secretome derived from CSE-exposed A549 cells caused respiratory endothelial cell death, inflammation, and moderately enhanced endothelial permeability. This study demonstrates the potential role of cellular interaction between endothelial and epithelial cells during exposure to CSE and provides novel therapeutic targets or beneficial biomarkers using secretome analysis for CSE-related respiratory diseases. ? The Author(s) 2024.

License

CC BY

Rights

Authors

Publication Source

WoS

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