• Self-enhancement lateral flow immunoassay for COVID-19 diagnosis

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Metadata

Document Title

Self-enhancement lateral flow immunoassay for COVID-19 diagnosis

Author

Ruantip S. Pimpitak U. Rengpipat S. Pasomsub E. Seepiban C. Gajanandana O. Torvorapanit P. Hirankarn N. Jaru-ampornpan P. Siwamogsatham S. Pongpaibool P. Siwamogsatham S. Thongchul N. Chaiyo S.

Affiliations

National Security and Dual-use Technology Center (NSD) National Science and Technology Development Agency (NSTDA) Pathum Thani12120 Thailand; Institute of Biotechnology and Genetic Engineering Chulalongkorn University Bangkok 10330 Thailand; Qualified Diagnostic Development Center (QDD) Chulalongkorn University Bangkok 10330 Thailand; Department of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University Bangkok Thailand; Monoclonal Antibody Production and Application Research Team National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani12120 Thailand; Thai Red Cross Emerging Infectious Diseases Clinical Center King Chulalongkorn Memorial Hospital Bangkok Thailand; Division of Infectious Disease Department of Medicine Faculty of Medicine Chulalongkorn University Bangkok Thailand; Center of Excellence in Immunology and Immune-Mediated Diseases Department of Microbiology Faculty of Medicine Chulalongkorn University Bangkok Thailand; Virology and Cell Technology Research Team National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani12120 Thailand; Division of Ambulatory and Hospital Medicine Department of Medicine Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand; Chula Clinical Research Center Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand; Research Unit in Bioconversion and Bioseparation for Value-Added Chemical Production Chulalongkorn University Bangkok 10330 Thailand; Center of Excellence for Food and Water Risk Analysis (FAWRA) Chulalongkorn University Bangkok 10330 Thailand

Type

Article

Source Title

Sensors and Actuators B: Chemical

ISSN

9254005

Year

2023

Volume

389

Open Access

All Open Access Bronze Green

Publisher

Elsevier B.V.

DOI

10.1016/j.snb.2023.133898

Abstract

Equipment-free colorimetric-based lateral flow immunoassay (LFIA) is the most convenient and popular tool for various applications including diagnostic tools requiring high sensitivity for the detection of pathogens. Thus improvements and developments of LFIA are constantly being reported. Herein we enriched the sensitivity of LFIA using the gold enhancement principle emphasizing needlessly complicated apparatus only one step for the strip test operation and typical time incubation (15 min) process. Self-enhanced LFIA was then executed for subsequent flows by overlapping the additionally enhanced pad composed of gold ions and reducing agent on the conjugate pad and the sample pad. Self-enhanced LFIA was performed to detect SARS-CoV-2 antigens in saliva. The obtained result depicted that the achieved sensitivity was up to tenfold compared with that of conventional LFIA by visual measurements. The detection limits of self-enhanced LFIA detecting nucleocapsid protein antigens in the saliva sample was 0.50 and 0.10 ng/mL employed by naked eye detection and calibration curve-based calculation respectively. When the proposed device was applied to 207 human saliva samples the diagnostic performance presented a 96.10 % sensitivity and 99.23 % specificity. This self-enhanced LFIA could be implemented in large-scale production and demonstrates higher sensitivity with effortless use which meets the requirements for point-of-care testing and on-field mass screening. ? 2023 Elsevier B.V.

Industrial Classification

N/A

Knowledge Taxonomy Level 1

Biological Sciences

Knowledge Taxonomy Level 2

Biological nanosensor technology

Knowledge Taxonomy Level 3

Biological nanosensor technology

License

Copyright

Rights

Elsevier B.V.

Publication Source

WOS

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