• Secretome profiling of human epithelial cells exposed to cigarette smoke extract and their effect on human lung microvascular endothelial cells

  • Back
06/12/2024 by   

Metadata

Document Title

Secretome profiling of human epithelial cells exposed to cigarette smoke extract and their effect on human lung microvascular endothelial cells

Author

Seenak P., Nernpermpisooth N., Kumphune S., Songjang W., Jiraviriyakul A., Jumroon N., Pankhong P., Roytrakul S., Thaisakun S., Phaonakrop N., Nuengchamnong N.

Affiliations

Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand; National Biobank of Thailand (NBT), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand; Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand; Frontier Research Center (FRC) Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand; Laboratory of Microbial Genetic Technology, Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, Fukuoka, 812-8581, Japan; Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok, 10110, Thailand; Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla, Hat Yai90110, Thailand

Type

Article

Source Title

Journal of King Saud University - Science

ISSN

10183647

Year

2024

Volume

36

Issue

4

Open Access

All Open Access, Gold

Publisher

Elsevier B.V.

DOI

10.1016/j.jksus.2024.103111

Abstract

Aeromonas dhakensis stands out as the most potent Aeromonas species causing a range of human diseases. This research marks the pioneering effort in isolating and characterizing virulent phages targeting A. dhakensis. Only the AM isolate among the Aeromonas isolates showed compatibility for phage isolation and was identified as A. dhakensis. Computational analysis identified the presence of virulence factors and antimicrobial resistance genes in A. dhakensis AM. Phage isolation was conducted using this particular strain as the host, resulting in the isolation of four virulent phages: vB_AdhM_DL, vB_AdhS_TS3, vB_AdhM_TS9, and vB_AdhS_M4. Bacterial numbers significantly decrease after both pre-treatment and post-treatment with individual phages and phage cocktails, ranging from 2.82 to 6.67 log CFU/mL and 4.01 to 6.49 log CFU/mL, respectively. Combining a phage cocktail with sub-MIC amoxicillin led to complete inactivation in both pre-treatment and post-treatment scenarios within a 200 ?L volume. The complete genomes of phages vB_AdhM_DL, vB_AdhS_TS3, and vB_AdhM_TS9 were determined to be 42,388 bp, 115,560 bp, and 115,503 bp, respectively. This study establishes the effectiveness of using phages as an complement with sublethal antibiotic concentrations, presenting a potential and effective therapeutic approach. ? 2024 The Author(s)

License

CC BY-NC-ND

Rights

Authors

Publication Source

WoS

Continue browsing