• Scorpion venom peptide effects on inhibiting proliferation and inducing apoptosis in canine mammary gland tumor cell lines

  • Back
 by   

Metadata

Document Title

Scorpion venom peptide effects on inhibiting proliferation and inducing apoptosis in canine mammary gland tumor cell lines

Author

Panja K.,Buranapraditkun S.,Roytrakul S.,Kovitvadhi A.,Lertwatcharasarakul P.,Nakagawa T.,Limmanont C.,Jaroensong T.

Name from Authors Collection

Panja K.

Scopus Author ID

57226001839

ORCID ID

NULL

|

Buranapraditkun S.

Scopus Author ID

6506892388

ORCID ID

NULL

|

Sittiruk Roytrakul

Scopus Author ID

6508382189

ORCID ID

0000-0003-3696-8390

|

Kovitvadhi A.

Scopus Author ID

56562974200

ORCID ID

NULL

|

Lertwatcharasarakul P.

Scopus Author ID

27867878500

ORCID ID

NULL

|

Nakagawa T.

Scopus Author ID

57233589400

ORCID ID

NULL

|

Limmanont C.

Scopus Author ID

55604670700

ORCID ID

NULL

|

Jaroensong T.

Scopus Author ID

35487520700

ORCID ID

NULL

Affiliations

Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkhen Campus, Bangkok, 10900, Thailand; Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Bangpra, Chonburi, 20110, Thailand; Division of Allergy and Clinical Immunology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center-Chula VRC), Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Thai Pediatric Gastroenterology, Hepatology and Immunology (TPGHAI) Research Unit, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand; Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand; Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kampaeng Saen Campus, Nakhon Pathom, 73140, Thailand; Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

Type

Article

Source Title

Animals

ISSN

20762615

Year

2021

Volume

11

Issue

7

Open Access

All Open Access, Gold, Green

Publisher

MDPI

DOI

10.3390/ani11072119

Abstract

The most common neoplasms in intact female dogs are CMGTs. BmKn-2, an antimicrobial peptide, is derived from scorpion venom and has published anticancer effects in oral and colon human cancer cell lines. Thus, it is highly likely that BmKn-2 could inhibit CMGT cell lines which has not been previously reported. This study investigated the proliferation and apoptotic properties of BmKn-2 via Bax and Bcl-2 relative gene expression in two CMGT cell lines, metastatic (CHMp-5b) and non-metastatic (CHMp-13a). The results showed that BmKn-2 inhibited proliferation and induced apoptosis in the CMGT cell lines. The cell morphology clearly changed and increased apoptosis in a dose dependent of manner. The half maximum inhibitory concentration (IC50) was 30 µg/mL for CHMp-5b cell line and 54 µg/mL for CHMp-13a cell line. The induction of apoptosis was mediated through Bcl-2 and Bax expression after BmKn-2 treatment. In conclusion, BmKn-2 inhibited proliferation and induced apoptosis in both CHMp-5b and CHMp-13a cell lines via down-regulation of Bcl-2 and up-regulation of Bax relative mRNA expression. Therefore, BmKn-2 could be feasible as candidate treatment for CMGTs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Industrial Classification

N/A

Knowledge Taxonomy Level 1

N/A

Knowledge Taxonomy Level 2

N/A

Knowledge Taxonomy Level 3

N/A

License

CC BY

Rights

Author

Publication Source

Scopus

Continue browsing