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Rice Sesquiterpene Plays Important Roles in Antixenosis against Brown Planthopper in Rice
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Metadata
Document Title
Rice Sesquiterpene Plays Important Roles in Antixenosis against Brown Planthopper in Rice
Author
Kamolsukyeunyong W, Sukhaket W, Pitija K, Thorngkham P, Mahatheeranont S, Toojinda T, Vanavichit A
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; Chiang Mai University; Kasetsart University; Kasetsart University
Type
Article
Source Title
PLANTS-BASEL
Year
2021
Volume
10
Issue
6
Page
-
Open Access
gold, Green Published
Publisher
MDPI
DOI
10.3390/plants10061049
Format
Abstract
The rice sesquiterpene synthase II gene (OsSTPS2, LOC_Os04g27430), which is involved in the antixenosis defense mechanism of rice against brown planthopper (BPH) infestation, was identified in the BPH-resistant rice variety Rathu Heenati (RH). In contrast, the gene was not functional in the BPH-susceptible rice variety KDML105 (KD). Single-nucleotide polymorphisms (SNPs) in the promoter region and in exon 5 of the gene and a seven amino acid deletion in the deduced protein sequence are suggested as factors that negatively regulate the function of the gene. Sequence analysis of the promoter region and expression analysis of the OsSTPS2 gene in several rice genotypes revealed the correlation of SNPs of the ATHB-1, SBE1, and P-factor with the expression of the gene. Genomic and complementary DNA (cDNA) sequence analysis at exon 5 of the gene showed that the 21 bp deletion naturally occurred in several rice genotypes. The antixenosis of the BPH feeding preference (AFP) of rice varieties differed in the seven amino acid deletion lesion of the gene, suggesting that the seven amino acid deletion negatively controls the antixenosis mechanism during BPH infestation. Analysis of the plant volatile compounds released after BPH infestation suggested that E-beta-farnesene (EBF) is the major product of the OsSTPS2 gene.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
National Science and Technology Development Agency (NSTDA) [P-18-52711]; Office of National Higher Education Science Research and Innovation Policy Council, Program Management Unit for Human Resources & Institutional Development, Research and Innovation [B16F630088]
License
CC BY
Rights
Authors
Publication Source
WOS