Protective effect of Anoectochilus burmannicus extracts and its active compound kinsenoside on adipocyte differentiation induced by benzyl butyl phthalate and bisphenol A
Protective effect of Anoectochilus burmannicus extracts and its active compound kinsenoside on adipocyte differentiation induced by benzyl butyl phthalate and bisphenol A
Author
Buacheen P. Karinchai J. Kammasit N. Temviriyanukul P. Butkinaree C. Watthana S. Wongnoppavich A. Imsumran A. Pitchakarn P.
Affiliations
Department of Biochemistry Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand; Institute of Nutrition Mahidol University Salaya Campus Nakhon Pathom 73170 Thailand; Food and Nutrition Academic and Research Cluster Institute of Nutrition Mahidol University Nakhon Pathom 73170 Thailand; National Omics Center National Science and Technology Development Agency Pathum Thani 12120 Thailand; School of Biology Institute of Science Suranaree University of Technology Nakhon Ratchasima 30000 Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2023
Volume
13
Issue
1
Open Access
All Open Access Gold Green
Publisher
Nature Research
DOI
10.1038/s41598-023-30227-5
Abstract
Benzyl butyl phthalate (BBP) and bisphenol-A (BPA) are obesogens that have been reported to be associated with obesity. Inhibition of their adipogenic activity could decrease the risk of obesity-related metabolic disorders. This study hypothesized that Anoectochilus burmannicus ethanolic extract (ABE) which has been previously reported its anti-inflammation property and its known active compound kinsenoside (Kin) abrogate BBP- and BPA-induced adipogenesis. ABE and Kin markedly suppress both BBP- and BPA-stimulated adipogenesis with different modulations on adipogenic-gene expression including C/EBP? PPAR? adiponectin and leptin in 3T3-L1. BBP induced C/EBP? adiponectin and leptin mRNA expressions and slightly increased PPAR? mRNA level whereas BPA markedly induced PPAR? and adiponectin mRNA levels. ABE significantly decreased the expression of C/EBP? and leptin but not PPAR? and adiponectin in the BBP-treated cells. In the BPA-treated cells ABE significantly decreased the mRNA expression of C/EBP? and PPAR? but not adiponectin and leptin. Interestingly Kin significantly overcame BBP- and BPA-induced C/EBP? PPAR? adiponectin and leptin expressions. This study first provides evidence to support the health benefits of this plant especially for people exposed to obesogens. Besides this finding would encourage the conservation and culture of this orchid for development as an economic plant and healthy food. ? 2023 The Author(s).
