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Production and Immunogenicity Assessment of LTp50: An Escherichia coli-Made Chimeric Antigen Targeting S1- and S2-Epitopes from the SARS-CoV-2/BA.5 Spike Protein
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Metadata
Document Title
Production and Immunogenicity Assessment of LTp50: An Escherichia coli-Made Chimeric Antigen Targeting S1- and S2-Epitopes from the SARS-CoV-2/BA.5 Spike Protein
Author
Wong-Arce A., Gonzalez-Ortega O., Romero-Maldonado A., Miranda-L?pez A., Garc?a-Soto M., Farf?n-Castro S., Betancourt-Mendiola L., Teeravechyan S., Srisutthisamphan K., Comas-Garc?a M., Sol?s Andrade K.I., Rosales-Mendoza S.
Affiliations
Department of Biotechnology, Faculty of Science and Technology, Thammasat University, Rangsit Campus, Pathum Thani, 12120, Thailand; Mitr Phol Innovation and Research Center, Chaiyaphum, 36110, Thailand; Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand; Northeast Thailand Cane and Sugar Research Center, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
Type
Article
Source Title
Plants
ISSN
22237747
Year
2024
Volume
13
Issue
8
Open Access
All Open Access, Gold
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/plants13081072
Abstract
Drought is a significant constraint to sugarcane productivity. Therefore, understanding how different varieties of sugarcane respond to drought stress can facilitate breeding programs and set up criteria for selecting drought-tolerant varieties. In the present study, we examined eight morpho-physiological traits to distinguish 40 sugarcane genotypes categorized into four groups based on significant differences in cane yield under non-stressed conditions and reduction of cane yield under drought-stressed conditions. The study was conducted during the formative stage in a greenhouse, encompassing both control and drought conditions. Drought treatments resulted in significant changes and differences in the mean values of various morpho-physiological traits. The hierarchical clustering analysis, utilizing stay-green traits such as higher chlorophyll fluorescence ratio (Fv/Fm), leaf chlorophyll content (SPAD), leaf relative water content (RWC), and lower leaf rolling score (LR), leaf drying score (LD), and drought recovery score (DR), successfully grouped 40 sugarcane genotypes into four major clusters, similar to the previously categorized groups. Correlation analysis showed significant relationships among cane yield, reduction of cane yield under drought conditions, and the stay-green traits. Our results demonstrated that morpho-physiological traits contributing to the “stay-green” phenotypes could be useful as selection criteria for drought tolerance in sugarcane. ? 2024 by the authors.
Keyword
Drought tolerance | green | stay | Sugarcane
License
CC BY
Rights
Authors
Publication Source
WoS