-
Phase II prefusion non-stabilised Covid-19 mRNA vaccine randomised study
- Back
Metadata
Document Title
Phase II prefusion non-stabilised Covid-19 mRNA vaccine randomised study
Author
Puthanakit T., Prompetchara E., Gatechompol S., Ketloy C., Thitithanyanont A., Jongkaewwattana A., Buranapraditkun S., Ubolyam S., Kerr S.J., Sophonphan J., Apornpong T., Kittanamongkolchai W., Siwamogsatham S., Sriplienchan S., Patarakul K., Theerawit T., Promsena P., Nantanee R., Manomaisantiphap S., Chokyakorn S., Hong L., Samija M., Montefiori D.C., Gao H., Eaton A., Wijagkanalan W., Alameh M.-G., Weissman D., Ruxrungtham K., Tawan M., Sutjarit A., Meepuksom T., Athipunjapong J., Jupimai T., Moonwong J., Nadsasarn R., Khamthi S., Nuncharoen P., Chanpoom Y., Khamkhen P., Narupan N., Thongthip S., Soisoongnern K., Shanyip C., Rachpradit T., Sriphraram K., Somhanwong W., Boondamnern T., Boonnak N., Chomchey N., Tipsuk S., Puttamaswin S., Yewande S., Lertarom P., Uanithirat A., Anuchadbut A., Chanthaburanun S., Tarawat K., Mahanontharit A., Sinthon W., Plakunmonthonw S., Wongmueang S., Dalodom T., Sopa B., Phongam N., Sri-Arunsak A., Chobkarching U., Bouko C., Junseeha S., Phuphalicho B., Pingthaisong P., Khlaiphuengsin A., Pararit P., Eamyoung P., Somjit T., Iampornsin T., Thongchomphunut D., Manopwisedjaroen S., Laopanupong T., Ekronarongchai S., Srisaowakarn C., Jantraphakorn Y., Srisutthisamphan K., Grandin P.V.
Affiliations
Department of Biochemistry, Center of Excellence for Molecular Biology and Genomics of Shrimp, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Biochemistry, Center of Excellence in Molecular Crop, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Biomedical Materials and Devices for Revolutionary Integrative Systems Engineering Research Unit (BMD-RISE), Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand; Nano Engineering Program, International School of Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Khlong Luang, 12120, Thailand; Department of Biomedical Science, BK21 FOUR Program in Biomedical Science and Engineering, Inha University College of Medicine, Incheon, 22332, South Korea; Biomedical Engineering Program, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; Biomedical Engineering Research Center, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2024
Volume
14
Issue
1
Open Access
All Open Access, Gold
Publisher
Nature Research
DOI
10.1038/s41598-024-53689-7
Abstract
Controlled release of proteins, such as growth factors, from biocompatible silk fibroin (SF) hydrogel is valuable for its use in tissue engineering, drug delivery, and other biological systems. To achieve this, we introduced silk fibroin-mimetic peptides (SFMPs) with the repeating unit (GAGAGS)n. Using green fluorescent protein (GFP) as a model protein, our results showed that SFMPs did not affect the GFP function when conjugated to it. The SFMP-GFP conjugates incorporated into SF hydrogel did not change the gelation time and allowed for controlled release of the GFP. By varying the length of SFMPs, we were able to modulate the release rate, with longer SFMPs resulting in a slower release, both in water at room temperature and PBS at 37 ?C. Furthermore, the SF hydrogel with the SFMPs showed greater strength and stiffness. The increased ?-sheet fraction of the SF hydrogel, as revealed by FTIR analysis, explained the gel properties and protein release behavior. Our results suggest that the SFMPs effectively control protein release from SF hydrogel, with the potential to enhance its mechanical stability. The ability to modulate release rates by varying the SFMP length will benefit personalized and controlled protein delivery in various systems. ? The Author(s) 2024.
License
CC BY
Rights
Authors
Publication Source
WoS