• Novel Serum Proteomes Expressed from Benzene Exposure Among Gasoline Station Attendants

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06/12/2024 by   

Metadata

Document Title

Novel Serum Proteomes Expressed from Benzene Exposure Among Gasoline Station Attendants

Author

Polyong C.P., Roytrakul S., Sirivarasai J., Yingratanasuk T., Thetkathuek A.

Affiliations

Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand; The Metabolomics Innovation Centre, University of Alberta, Edmonton, AB, Canada; Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand; Department of Chemistry, University of Alberta, Edmonton, AB, Canada; Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence for Companion Animal Cancer, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

Type

Article

Source Title

Journal of Veterinary Internal Medicine

ISSN

8916640

Year

2024

Open Access

All Open Access, Gold

Publisher

John Wiley and Sons Inc

DOI

10.1111/jvim.17092

Abstract

Background: Oral melanoma (OM) and oral squamous cell carcinoma (OSCC) are frequently diagnosed in dogs, presenting a challenge in distinguishing them from benign oral tumors (BN). Salivary metabolomic biomarkers offer a practical solution because of salivas direct contact with tumors and the noninvasive nature of collection. Objective: Assess the diversity and abundance of the salivary metabolome in dogs with BN, OM, and OSCC using amine/phenol submetabolome analysis and high-performance chemical isotope labeling liquid chromatography–mass spectrometry (CIL LC-MS). Animals: Study included 11 BN, 24 OM, 10 OSCC, and 20 healthy control dogs. Methods: Case-control cross-sectional study was conducted to assess salivary submetabolic profiles in dogs with BN, OM, and OSCC and healthy dogs. Samples were labeled with 12C-dansyl chloride and analyzed using CIL LC-MS targeted to amine- and phenol-containing metabolites for amine/phenol submetabolome analysis. Results: Distinct clusters and significant differences in metabolite concentrations were observed among the oral cancer, BN, and control groups. A total of 154 and 66 metabolites showed significantly altered concentrations, particularly in OM and OSCC, respectively, when compared with BN (Padj <.05). Potential metabolic biomarkers were identified for each cancer, including decreased concentrations of seryl-arginine and sarcosine in OSCC. Moreover, high-confidence putative metabolites were identified, including an increase in tryptophyl-threonine and a decrease in 1,2-dihydroxynapthalene-6-sulfonic acid and hydroxyprolyl-hydroxyproline for OM. Conclusions and Clinical Importance: We identified high coverage of the amine/phenol submetabolome, including seryl-arginine, and sarcosine, in OSCC. Our findings emphasize the potential of these biomarkers for distinguishing between oral OSCC and BN in dogs. ? 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.

License

CC BY-NC-ND

Rights

Authors

Publication Source

WoS

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