Abstract
A novel endophytic actinomycete, designated strain SC1-1(T), was isolated from sterilized stem tissue from Stahlianthus campanulatus collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented meso-diaminopimelic acid in the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose and ribose were observed as sugars in the cells. The cell membrane phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and ninhydrin-positive phosphoglycolipids. The major menaquinones were MK-9(H-4) and MK-9 (H-2). The main cellular fatty acids were iso-C-16:0, 10-methyl C-17:0 and C-17:1 omega 6c. A high G+C content (70.7 mol%) was present in the genomic DNA. The taxonomic position based on the 16S rRNA gene sequence analysis revealed that strain SC1-1T belonged to the genus Nonomuraea and shared the highest 16S rRNA gene sequence similarity value with Nonomuraea dietziae DSM 44320(T) (98.82 %), followed by Nonomuraea africana IFO 14745(T) (98.58 %), Nonomuraea jabiensis A4036(T) (98.43 %), Nonomuraea endophytica YIM 65601(T) (98.36 %), Nonomuraea purpurea 1SM4-01(T) (98.34 %), Nonomuraea angiospora IFO 13155(T) (98.29 %), Nonomuraea roseola IFO 14685(T) (98.23 %) and Nonomuraea recticatena IFO 14525(T) (98.23 %). On the basis of the DNA-DNA hybridization relatedness and including the physiological and biochemical characteristics, strain SC1-1(T) should be judged as a novel species of the genus Nonomuraea, for which the name Nonomuraea stahlianthi sp. nov. is proposed. The type strain is strain SC1-1(T) (= BCC 66361(T) = NBRC 110006(T)).
