Abstract
The novel actinomycete strain PWB-003(T), which produced fluostatins B and C antibiotics, was isolated from nearshore sediment collected from Panwa Cape, Phuket Province, Thailand. Data from the present polyphasic study indicated that strain PWB-003(T) represented a member of the genus Micromonospora. It produced single spores on substrate mycelia and contained mesodiaminopimelic acid in the cell-wall peptidoglycan. Whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. The predominant menaquinone was MK-10 (H-4). Cellular fatty acids comprised C-18 : 1 omega 9c, iso-C-16 : 0 anteiso-C-17 : (0), iso-C-15 : 0 and iso-C-17 : 0. On the basis of 16S rRNA gene sequence similarity analysis, the novel strain was closely related to Micromonospora eburnea LK2-10(T) (99.38 %), Micromonospora chaiyaphumensis MC5-1(T) (99.16 %), Micromonospora yangpuensis FXJ6.011(T) (98.97 %), Micromonospora echinaurantiaca DSM 43904(T) (98.97 %), Micromonospora pallida DSM 43817(T) (98.97 %), Micromonospora sagamiensis DSM 43912(T) and Micromonospora auratinigra JCM 12357(T) (both 98.97 %). The G+C content of the DNA was 74.5 mol%. DNA-DNA relatedness values among strain PWB-003(T) and related type strains ranged from 11.3 +/- 1.3 to 38.8 +/- 1.1 %. On the basis of these observations, strain PWB-003(T) could be distinguished from its closely related type strains and is considered to represent a novel species of the genus Micromonospora, for which the name Micromonospora fluostatini sp. nov. (type strain PWB-003(T)=JCM 30529(T)=PCU 341(T)=TISTR 2345(T)) is proposed.
