MGI short-read genome assemblies of Pythium insidiosum (reclassified as Pythium periculosum) strains Pi057C3 and Pi050C3

Abstract

Objectives: Pythium insidiosum causes a difficult-to-treat infectious condition called pythiosis with high morbidity and mortality. So far genome data of at least 10 strains of P. insidiosum primarily classified in the phylogenetic clades I and II have been sequenced using various next-generation sequencing platforms. The MGI short-read platform was employed to obtain genome data of 2 clade-III strains of P. insidiosum (recently reclassified as Pythium periculosum) from patients in Thailand and the United States. This work is a part of our attempt to generate a comprehensive genome database from diverse pathogen strains. Data description: A 150-bp paired-end library was prepared from a gDNA sample of P. insidiosum (P. periculosum) strains Pi057C3 and Pi050C3 (also known as ATCC90586) to generate draft genome sequences using an MGISEQ-2000RS sequencer. As a result for the strain Pi057C3 we obtained a 42.5-Mb assembled genome (164x coverage) comprising 14 134 contigs L50 of 241 N50 of 45 748 57.6% CG content and 12 147 ORFs. For the strain Pi050C3 we received a 43.3-Mb draft genome (230x coverage) containing 14 511 contigs L50 of 245 N50 of 45 208 57.7% CG content and 12 249 ORFs. The genome sequences have been deposited in the NCBI/DDBJ databases under the accession numbers JAKCXM000000000.1 (strain Pi057C3) and JAKCXL000000000.1 (strain Pi050C3). ? 2023 The Author(s).