Kasetsart University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); National Institute of Agrobiological Sciences - Japan; National Institute of Agrobiological Sciences - Japan; Kasetsart University
Type
Article
Source Title
DNA RESEARCH
ISSN
1340-2838
Year
2001
Volume
8
Issue
4
Page
163-171
Open Access
gold
Publisher
UNIVERSAL ACADEMY PRESS INC
DOI
10.1093/dnares/8.4.163
Format
PDF
Abstract
The major QTL for submergence tolerance was locate in the 5.9 cM interval between flanking RFLP markers. To narrow down this region, a physical map was constructed using YAC and BAC clones. A 400-kb YAC was identified in this region and later its end fragments were used to screen a rice BAC library. Through chromosome walking, 24 positive BAC clones formed two contigs around linked-RFLP markers, R1164 and RZ698. Using one YAC end, six BAC ends and three RFLP markers, a fine-scale map was constructed of the 6.8-cM interval of S10709-RZ698 on rice chromosome 9. The submergence tolerance and related trait were located in a small, well-defined region around BAC-end marker 180D1R and RFLP marker R1164. The physical-to-map distance ratio in this region is as small as 172.5 kb/cM, showing that this region is a hot spot for recombination in the rice genome.
