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Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform
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Metadata
Document Title
Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform
Author
Inlumphan S.
Name from Authors Collection
Affiliations
College of Materials Innovation and Technology, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok, 10520, Thailand; National Nanotechnology Center (NANOTEC), NSTDA, 111 Thailand Science Park, Paholyothin Rd., Klong Luang, Pathum Thani, 12120, Thailand; Center of Excellence in Polymeric Materials for Medical Practice Devices, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand; Interdisciplinary Cluster for Cutting Edge Research, Research Initiative for Supra-Materials, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan; Global Aqua Innovation Center, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan; Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan; National Electronics and Computer Technology Center (NECTEC), NSTDA, 111 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani, 12120, Thailand; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand
Type
Article
Source Title
Sensors and Actuators Reports
ISSN
26660539
Year
2025
Volume
9
Open Access
All Open Access; Gold Open Access; Green Open Access
Publisher
Elsevier B.V.
DOI
10.1016/j.snr.2024.100276
Abstract
Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO2 laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening. © 2024 The Author(s)
Keyword
Dengue virus | electrochemical sensor | Immunosensor | Laser-induced graphene | Serology
Industrial Classification
License
CC BY-NC-ND
Rights
Authors
Publication Source
Scopus