Abstract
A novel actinomycete strain, SMC 256(T), which developed small, globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from soil collected in a mountain forest of Thailand. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SMC 256(T) belonged to the genus Kutzneria, and the closest phylogenetically related species were Kutzneria buriramensis BCC 29373(T) (98.9 % 16S rRNA gene sequence similarity), Kutzneria kofuensis ATCC 27102(T) (98.2 %), Kutzneria albida ATCC 25243(T) (97.9 %) and Kutzneria viridogrisea ATCC 25242(T) (97.4 %). The DNA-DNA relatedness values that distinguished strain SMC 256(T) from previously described members of the genus Kutzneria were significantly below 70 %. The G+C content of the genomic DNA was 71.8 mol%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars consisted of rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H-4). Mycolic acids were not detected. The diagnostic phospholipids were hydroxyphosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unidentified phosphoglycolipids, unidentified phospholipids and an unidentified lipid. The predominant cellular fatty acids were iso-C-16 : 0, C-17 : 1 and C-17 : 0 10-methyl. Following the evidence of phenotypic, chemotaxonomic and genotypic studies, it is proposed that strain SMC 256(T) represents a novel species in the genus Kutzneria, namely Kutzneria chonburiensis sp. nov. The type strain is SMC 256(T) (=BCC 72675(T) =NBRC 110610(T)).
