Abstract
Thermotolerant bacterial nanocellulose-producing strains, designated MSKU 9(T) and MSKU 15, were isolated from persimmon and sapodilla fruits, respectively. These strains were aerobic, Gram-stain-negative, had rod-shaped cells, were non-motile and formed white-cream colonies. Phylogeny based on the 16S rRNA gene sequences revealed that MSKU 9(T) and MSKU 15 represented members of the genus Komagataeibacter and formed a monophyletic branch with K. swingsii JCM 17123(T) and K. europaeus DSM 6160(T). The genomic analysis revealed that overall genomic relatedness index values of MSKU 9(T) with K. swingsii JCM 17123(T) and K. europaeus DSM 6160T were similar to 90% average nucleotide identity (ANI) and <= 58.2 % digital DNA-DNA hybridization (dDDH), respectively. MSKU 9(T) and MSKU 15 can be differentiated from the closely related K. swingsii JCM 17123(T) by their growth on 30% d-glucose and ability to utilize and to form acid from raffinose and sucrose as carbon sources, and from K. europaeus DSM 6160(T) by their ability to grow without acetic acid. The genomic DNA G+C contents of MSKU 9(T) and MSKU 15 were 60.4 and 60.2 mol%, respectively. The major fatty acids of MSKU 9(T) and MSKU 15 were summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c). The respiratory quinone was determined to be Q10. On the basis of the results of the polyphasic taxonomic analysis, MSKU 9(T) (=TBRC 9844(T)=NBRC 113802(T)) represents a novel species of the genus Komagataeibacter, for which the name Komagataeibacter diospyri sp. nov. is proposed.
