• Investigation of Plasma Cell-Free DNA and MiRNA in Cholangiocarcinoma and Opisthorchiasis Viverrini Patients

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06/12/2024 by   

Metadata

Document Title

Investigation of Plasma Cell-Free DNA and MiRNA in Cholangiocarcinoma and Opisthorchiasis Viverrini Patients

Author

Prasopdee S., Pholhelm M., Yusuk S., Tangphatsornruang S., Butthongkomvong K., Kunjantarachot A., Phanaksri T., Kulsantiwong J., Tesana S., Thitapakorn V.

Affiliations

Aquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Coastal Aquaculture Technology and Innovation Research and Development Center, Department of Fisheries, Ministry of Agriculture and Cooperative, Songkhla, 80170, Thailand; Faculty of Veterinary Medicine, Rajamangala University of Technology Srivijaya, Songkhla, 90000, Thailand; Faculty of Innovative Agriculture and Fishery Establishment Project, Prince of Songkla University, Surat Thani Campus, Surat Thani, 84000, Thailand; Department of Fisheries, Ministry of Agriculture and Cooperative, Chatuchak, Bangkok, 10900, Thailand; Department of Aquatic Biosciences, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, Japan

Type

Article

Source Title

Aquaculture and Fisheries

ISSN

20961758

Year

2024

Open Access

All Open Access, Gold

Publisher

KeAi Communications Co.

DOI

10.1016/j.aaf.2024.04.006

Abstract

To establish a new breeding program, genetic diversity of five domesticated populations and two commercial stocks of Asian seabass (Lates calcarifer) were examined. Moderate levels of genetic diversity based on microsatellite polymorphism were found. The number of alleles per locus (NA) was 3.429–11.571. Observed (Ho) and expected (He) heterozygosities were 0.416–0.772 and 0.423–0.805. FST and genetic heterogeneity analysis revealed significant genetic differences of most pairwise comparisons (P < 0.001). Genetic distance between populations was 0.000–0.703. Phylogenetic analysis divided examined populations to two genetic stocks; A: DOF-Chachoengsao 2, DOF-Songkhla, and DOF-Trang and B: Commercial Farm 1 (Phuket) and Commercial Farm 2 (Chachengsao), DOF-Chachoengsao 1 and DOF-Krabi. The base population (G0, 51 families) was established from crosses between these founder populations. Heritability and estimated breeding values (EBVs) for growth in G0 families were evaluated at 519 day post hatch (dph, mean body weight = 616.64 ? 266.80 g, N = 1655). The heritability (h2) for growth of L. calcarifer in this study was 0.386 ? 0.020. The additive genetic coefficient of variation was 28% indicating high potential of genetic improvement for growth in this population. The distribution of EBVs showed high variation both between and within families. Fish exhibiting high EBVs (SATREPS-DOF-G0-hEBVs) clustered with a phylogenetic clade A while the SATREPS-DOF-G0-lEBVs group was phylogenetically allocated to clade B. Genetic selection with +1SD criteria is expected to achieve genetic gain of 28.5% per generation. The information on genetic diversity and EBVs estimates allows efficient implementation of our ongoing breeding program of L. calcarifer. ? 2024 Shanghai Ocean University.

License

CC BY-NC-ND

Rights

Shanghai Ocean University

Publication Source

Scopus

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