• Integrated lateral flow immunoassays using trimethylsilyl cellulose barriers for the enhanced sensitivity of COVID-19 diagnosis

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Metadata

Document Title

Integrated lateral flow immunoassays using trimethylsilyl cellulose barriers for the enhanced sensitivity of COVID-19 diagnosis

Author

Kumsab J. Deenin W. Yakoh A. Pimpitak U. Amornkitbamrung L. Rengpipat S. Hirankarn N. Crespo G.A. Chaiyo S.

Affiliations

The institute of Biotechnology and Genetic Engineering Chulalongkorn University Bangkok 10330 Thailand; Proteomics Research Team National Omics Center (NOC) National Science and Technology Development Agency (NSTDA) Pathum Thani 12120 Thailand; Program in Biotechnology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Center of Excellence for Food and Water Risk Analysis (FAWRA) Department of Veterinary Public Health Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand; Sensor Research Unit Department of Chemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Qualified Diagnostic Development Center Chulalongkorn University Bangkok 10330 Thailand; Department of Microbiology Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand; Department of Chemistry School of Engineering Sciences in Chemistry Biotechnology and Health KTH Royal Institute of Technology Teknikringen 30 Stockholm SE-100 44 Sweden; UCAM SENS UCAM-SENS Universidad Cat?lica San Antonio de Murcia UCAM HiTech Murcia 30107 Spain

Type

Article

Source Title

Journal of Science: Advanced Materials and Devices

ISSN

24682284

Year

2023

Volume

8

Issue

4

Open Access

All Open Access Gold

Publisher

Elsevier B.V.

DOI

10.1016/j.jsamd.2023.100620

Abstract

Lateral flow immunoassays (LFIAs) serve as rapid and convenient tools for disease screening. However there is room for enhancing diagnostic sensitivity. Therefore this paper introduces a novel material combined with lateral flow immunoassays to improve the sensitivity of COVID-19 detection and diagnosis. Trimethylsilyl cellulose (TMSC) was printed on the device between the test and control line to delay the flow of the solution which resulted in a more excellent binding of the immunoassay. These delay-LFIAs (d-LFIAs) were developed for the diagnosis of COVID-19 targeting the detection of immunoglobulins G and M as well as the COVID-19 antigen. Under optimized conditions the d-LFIA increased the sensitivity by up to 5 times compared to that of conventional LFIAs. Moreover it exhibited the ability to detect IgG IgM and SARS-CoV-2 antigens as low as 1 ng/mL (limit of detection) by the naked eye. In addition the application of this device to real samples yielded highly consistent results with those obtained from other LFIAs and standard SARS-CoV-2 diagnostic methods (such as enzyme-linked immunosorbent assay and reverse transcription polymerase chain reaction) diagnosis. Therefore we expect this device to be a new platform for highly sensitive easy and affordable immunoassay strip testing to certify future emerging diseases. ? 2023 Vietnam National University Hanoi

License

CC BY-NC-ND

Rights

Authors

Publication Source

WOS

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