Purdue University System; Purdue University; Purdue University West Lafayette Campus; Chiang Mai University; Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University
Type
Article
Source Title
JOURNAL OF VIROLOGY
Year
2010
Volume
84
Issue
16
Page
8353-8358
Open Access
Green Published
Publisher
AMER SOC MICROBIOLOGY
DOI
10.1128/JVI.00696-10
Format
PDF
Abstract
During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.
Thailand Tropical Diseases Research Program [02-2-DEN-03-003]; Medical Biotechnology Network, National Center for Genetic Engineering and Biotechnology, Thailand; NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P01AI045976] Funding Source: NIH RePORTER
