Stanford University; National Science & Technology Development Agency - Thailand; National Electronics & Computer Technology Center (NECTEC); Stanford University; Howard Hughes Medical Institute; Stanford University
Type
Article
Source Title
OPTICS LETTERS
ISSN
0146-9592
Year
2009
Volume
34
Issue
15
Page
2309-2311
Open Access
Green Accepted
Publisher
OPTICAL SOC AMER
DOI
10.1364/OL.34.002309
Format
PDF
Abstract
We present a two-photon microscope that is approximately 2.9 g in mass and 2.0 x 1.9 x 1.1 cm(3) in size and based on a microelectromechanical systems (MEMS) laser-scanning mirror. The microscope has a focusing motor and a micro-optical assembly composed of four gradient refractive index lenses and a dichroic microprism. Fluorescence is captured without the detected emissions reflecting off the MEMS mirror, by use of separate optical fibers for fluorescence collection and delivery of ultrashort excitation pulses. Using this microscope we imaged neocortical microvasculature and tracked the flow of erythrocytes in live mice. (C) 2009 Optical Society of America
Office of Naval Research (ONR); National Science Foundation (NSF); NSF Center for Biophotonics; National Institutes of Health (NIH) Nanomedicine Development Center for Optical Control of Biological Function; Packard and Beckman Foundations; Stanford Graduate Research fellowships; NECTEC; NATIONAL EYE INSTITUTE [PN2EY018241] Funding Source: NIH RePORTER
