Impact of Wooden Breast myopathy on in vitro protein digestibility metabolomic profile and cell cytotoxicity of cooked chicken breast meat
Author
Trithavisup T. Krobthong S. Yingchutrakul Y. Sanpinit P. Malila Y.
Affiliations
National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani 12120 Thailand; Center of Excellence in Natural Products Chemistry (CENP) Department of Chemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand
Type
Article
Source Title
Poultry Science
ISSN
325791
Year
2024
Volume
103
Issue
1
Open Access
All Open Access Gold Green
Publisher
Elsevier Inc.
DOI
10.1016/j.psj.2023.103261
Abstract
This study investigated the impacts of Wooden Breast (WB) abnormality on in vitro protein digestibility and cytotoxicity of cooked chicken breast meat. Chicken breasts without (non-WB n = 6) or with severe WB condition (WB n = 6) were cooked and subjected to static in vitro protein digestion. The results showed no significant differences in free-NH2 degree of hydrolysis and distribution of peptide molecular weight between non-WB and WB samples at late intestinal digestion (P5) suggesting no adverse effects of WB on protein digestibility. Based on peptidomic analysis P5 fraction of WB showed greater content of peptides with oxidative modification than that of non-WB. Untargeted metabolomics did not find any metabolites with potential toxicity either in non-WB and WB. Hydrolyzed non-WB and WB (1.56�0 ?g/mL) did not affect viability of Caco-2 and Vero cells but addition of WB samples reduced Caco-2 cell viability compared with non-WB. ? 2023 The Authors
