Mahidol University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University; Academia Sinica - Taiwan; Academia Sinica - Taiwan; National Taiwan University; Chiang Mai University
Type
Article
Source Title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Year
2008
Volume
372
Issue
1
Page
67-72
Open Access
Green Published
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI
10.1016/j.bbrc.2008.04.165
Format
PDF
Abstract
Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells. (C) 2008 Elsevier Inc. All rights reserved.
