• Hyperbaric oxygen therapy exerts anti-osteoporotic effects in obese and lean D-galactose-induced aged rats

  • Back
 by   

Metadata

Document Title

Hyperbaric oxygen therapy exerts anti-osteoporotic effects in obese and lean D-galactose-induced aged rats

Author

Imerb N. Thonusin C. Pratchayasakul W. Chanpaisaeng K. Aeimlapa R. Charoenphandhu N. Chattipakorn N. Chattipakorn S.C.

Affiliations

Neurophysiology Unit Cardiac Electrophysiology Research and Training Center Faculty of Medicine Chiang Mai University Chiang Mai Thailand; Center of Excellence in Cardiac Electrophysiology Faculty of Medicine Chiang Mai University Chiang Mai Thailand; Department of Oral and Maxillofacial Surgery Faculty of Dentistry Chiang Mai University Chiang Mai Thailand; Department of Physiology Faculty of Medicine Chiang Mai University Chiang Mai Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) Pathum Thani Thailand; Center of Calcium and Bone Research (COCAB) Faculty of Science Mahidol University Bangkok Thailand; Department of Physiology Faculty of Science Mahidol University Bangkok Thailand; Institute of Molecular Biosciences Mahidol University Nakhon Pathom Thailand; The Academy of Science The Royal Society of Thailand Bangkok Thailand; Department of Oral Biology and Diagnostic Sciences Faculty of Dentistry Chiang Mai University Chiang Mai Thailand

Type

Article

Source Title

FASEB Journal

ISSN

8926638

Year

2023

Volume

37

Issue

11

Open Access

All Open Access Bronze

Publisher

John Wiley and Sons Inc

DOI

10.1096/fj.202301197RR

Abstract

Obesity accelerates the aging processes resulting in an aggravation of aging-induced osteoporosis. We investigated the anti-osteoporotic effect of hyperbaric oxygen therapy (HBOT) in obese- and lean-aged rats through measurement of cellular senescence hypoxia inflammation antioxidants and bone microarchitecture. Obese and lean male Wistar rats were injected with 150 mg/kg/day of D-galactose for 8 weeks to induce aging. Then all rats were randomly given either sham or HBOT for 14 days. Metabolic parameters were determined. Expression by bone mRNA for cellular senescence hypoxia inflammation antioxidative capacity and bone remodeling were examined. Micro-computed tomography and atomic absorption spectroscopy were performed to evaluate bone microarchitecture and bone mineral profiles respectively. We found that HBOT restored the alterations in the mRNA expression level of p16 p21 HIF-1? TNF-? IL-6 RANKL RANK NFATc1 DC朣TAMP Osx ALP and Col1a1 in the bone in obese-and lean- aging rats. In obese-aging rats HBOT increased the level of expression of Sirt1 and CuZnSOD mRNA and diminished the expression level of HIF-2? and ctsk mRNA to the same levels as the control group. However HBOT failed to alter catalase and OCN mRNA expression in obese-aged rats. HBOT partially improved the bone microarchitecture in obese-aged rats but completely restored it in lean-aged rats. Interestingly HBOT protected against obesity-induced demineralization in obese-aged rats. In summary HBOT exerts an anti-osteoporotic effect in lean-aged rats and prevents some but not all the negative effects of obese-aged conditions on bone health. Therefore HBOT is considered as a potential therapy for aging-induced osteoporosis regardless of obese status. ? 2023 Federation of American Societies for Experimental Biology.

Continue browsing