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Genomic evidence supporting the clonal expansion of extensively drug-resistant tuberculosis bacteria belonging to a rare proto-Beijing genotype
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Metadata
Document Title
Genomic evidence supporting the clonal expansion of extensively drug-resistant tuberculosis bacteria belonging to a rare proto-Beijing genotype
Author
Srilohasin P.,Prammananan T.,Faksri K.,Phelan J.E.,Suriyaphol P.,Kamolwat P.,Smithtikarn S.,Disratthakit A.,Regmi S.M.,Leechawengwongs M.,Twee-Hee Ong R.,Teo Y.Y.,Tongsima S.,Clark T.G.,Chaiprasert A.
Name from Authors Collection
Affiliations
Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases (RCEID), Khon Kaen University, Khon Kaen, Thailand; Faculty of Infectious and Tropical Diseases, London School of Hygiene Tropical Medicine, London, United Kingdom; Division of Bioinformatics and Data Management for Research, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Research Group and Research Network Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand; Bureau of Tuberculosis, Department of Disease Control, Ministry of Public Health, Bangkok, Thailand; Department of Microbiology, Gandaki Medical College Teaching Hospital, Pokhara, Nepal; Vichaiyut Hospital, Bangkok, Thailand; Saw Swee Hock School of Public Health, National University of Singapore, National University Health System, Singapore, Singapore; National Biobank of Thailand, National Science and Technology Development Agency, Pathum Thani, Thailand; Faculty of Epidemiology and Population Health, London School of Hygiene Tropical Medicine, London, United Kingdom
Type
Article
Source Title
Emerging Microbes and Infections
ISSN
22221751
Year
2020
Volume
9
Issue
1
Page
2632-2641
Open Access
All Open Access, Gold, Green
Publisher
Taylor and Francis Ltd.
DOI
10.1080/22221751.2020.1852891
Abstract
Tuberculosis disease (TB), caused by Mycobacterium tuberculosis, is a major public health issue in Thailand. The high prevalence of modern Beijing (Lineage 2.2.1) strains has been associated with multi- and extensively drug-resistant infections (MDR-, XDR-TB), complicating disease control. The impact of rarer proto-Beijing (L2.1) strains is less clear. In our study of thirty-seven L2.1 clinical isolates spanning thirteen years, we found a high prevalence of XDR-TB cases (32.4%). With ≤ 12 pairwise SNP distances, 43.2% of L2.1 patients belong to MDR-TB or XDR-TB transmission clusters suggesting a high level of clonal expansion across four Thai provinces. All XDR-TB (100%) were likely due to transmission rather than inadequate treatment. We found a 47 mutation signature and a partial deletion of the fadD14 gene in the circulating XDR-TB cluster, which can be used for surveillance of this rare and resilient M. tuberculosis strain-type that is causing increasing health burden. We also detected three novel deletion positions, a deletion of 1285 bp within desA3 (Rv3230c), large deletions in the plcB, plcA, and ppe38 gene which may play a role in the virulence, pathogenesis or evolution of the L2.1 strain-type. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus