National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); University of Arkansas System; University of Arkansas Medical Sciences; Mahidol University; University of Arkansas System; University of Arkansas Fayetteville; University of Arkansas Little Rock; University of Arkansas System; University of Arkansas Medical Sciences; Thammasat University; Chandrakasem Rajabhat University
Type
Article
Source Title
INFECTION GENETICS AND EVOLUTION
Year
2021
Volume
87
Open Access
Green Accepted
Publisher
ELSEVIER
DOI
10.1016/j.meegid.2020.104674
Format
PDF
Abstract
Streptococcus suis, a zoonotic bacterial pathogen, has negative economic impacts on both intensive swine production and human health worldwide. Whole-genome sequencing and comparative genomic analysis have been widely used for comprehensive classification and investigation of the genetic basis of several S. suis strains obtained from distinct hosts in different geographic areas, revealing great genetic diversity of this zoonotic pathogen. In this study, whole-genome sequences of antibiotic-resistant S. suis strains isolated from human patients (2 strains), diseased pigs (4 strains), and asymptomatic pigs (3 strains) in Thailand were compared with known genomes of 1186 S. suis strains. Single-nucleotide polymorphism-based phylogenetic analysis indicated that the Thai-isolated S. suis strains have close genetic relatedness to S. suis strains isolated from Canada, China, Denmark, Netherlands, United Kingdom, and United States of America. The genome analysis revealed genes conferring antibiotic resistance (aad(6), ant(6)-Ia, ermB, tet(O), patB, and sat4) and gene clusters (aph(3')-IIIa and aac(6')-Ieaph(2 '')-Ia) associated with aminoglycoside, macrolide, and fluoroquinolone resistance in S. suis in Thailand. This work provides additional resources for future genomic epidemiology investigation of S. suis.
BIOTEC-Research Initiative Grant for Food Biotechnology Research Unit (BIOTEC-RI) [P16-51-873]; National Institute of General Medical Sciences of the National Institutes of Health [P20GM125503]
