• Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques

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Document Title

Full-length 16S rDNA sequencing based on Oxford Nanopore Technologies revealed the association between gut-pharyngeal microbiota and tuberculosis in cynomolgus macaques

Author

Sawaswong V. Chanchaem P. Klomkliew P. Rotcheewaphan S. Meesawat S. Kemthong T. Kaewparuehaschai M. Noradechanon K. Ekatat M. Kanitpun R. Srilohasin P. Warit S. Chaiprasert A. Malaivijitnond S. Payungporn S.

Affiliations

Department of Biochemistry Center of Excellence in Systems Microbiology Faculty of Medicine Chulalongkorn University 1873 Rama IV Road Patumwan Bangkok 10330 Thailand; Department of Biochemistry Faculty of Science Mahidol University Bangkok 10400 Thailand; Department of Microbiology Faculty of Medicine Chulalongkorn University Bangkok 10330 Thailand; National Primate Research Center of Thailand Chulalongkorn University Saraburi 18110 Thailand; Department of Biology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Wildlife Conservation Office Department of National Parks Wildlife and Plant Conservation Bangkok 10900 Thailand; National Institute of Animal Health (NIAH) Bangkok 10900 Thailand; Office for Research Faculty of Medicine Siriraj Hospital Mahidol University Bangkok 10700 Thailand; Department of Microbiology Faculty of Medicine Siriraj Hospital Mahidol University Bangkok 10700 Thailand; Industrial Tuberculosis Team Industrial Medical Molecular Biotechnology Research Group National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Pathum Thani 12120 Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2024

Volume

14

Issue

1

Open Access

All Open Access Gold

Publisher

Nature Research

DOI

10.1038/s41598-024-53880-w

Abstract

Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (Mtbc) which develops from asymptomatic latent TB to active stages. The microbiome was purposed as a potential factor affecting TB pathogenesis but the study was limited. The present study explored the association between gut-pharyngeal microbiome and TB stages in cynomolgus macaques using the full-length 16S rDNA amplicon sequencing based on Oxford Nanopore Technologies. The total of 71 macaques was divided into TB (?) control TB (+) latent and TB (+) active groups. The differential abundance analysis showed that Haemophilus hemolyticus was decreased while Prevotella species were increased in the pharyngeal microbiome of TB (+) macaques. In addition Eubacterium coprostanoligenes in the gut was enriched in TB (+) macaques. Alteration of these bacteria might affect immune regulation and TB severity but details of mechanisms should be further explored and validated. In summary microbiota may be associated with host immune regulation and affect TB progression. The findings suggested the potential mechanisms of host-microbes interaction which may improve the understanding of the role of microbiota and help develop therapeutics for TB in the future. ? The Author(s) 2024.

Industrial Classification

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Knowledge Taxonomy Level 1

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Knowledge Taxonomy Level 2

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Knowledge Taxonomy Level 3

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License

CC BY

Rights

Authors

Publication Source

WOS

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