Exploring the Impact of Endophytic Fungus Aspergillus cejpii DMKU-R3G3 on Rice: Plant Growth Promotion and Molecular Insights through Proteomic Analysis
Exploring the Impact of Endophytic Fungus Aspergillus cejpii DMKU-R3G3 on Rice: Plant Growth Promotion and Molecular Insights through Proteomic Analysis
Author
Leetanasaksakul K., Roytrakul S., Kittisenachai S., Lohmaneeratana K., Jantasuriyarat C., Lueangjaroenkit P.
Affiliations
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand
Type
Article
Source Title
PeerJ
ISSN
21678359
Year
2024
Volume
12
Open Access
All Open Access, Gold
Publisher
PeerJ Inc.
DOI
10.7717/peerj.16595
Abstract
Background. Plasmodium falciparum possesses a cobalamin-dependent methionine synthase (MS). MS is putatively encoded by the PF3D7_1233700 gene, which is orthologous and syntenic in Plasmodium. However, its vulnerability as an antimalarial target has not been assessed. Methods. We edited the PF3D7_1233700 and PF3D7_0417200 (dihydrofolate reductase-thymidylate synthase, DHFR-TS) genes and obtained transgenic P. falciparum parasites expressing epitope-tagged target proteins under the control of the glmS ribozyme. Conditional loss-of-function mutants were obtained by treating transgenic parasites with glucosamine. Results. DHFR-TS, but not MS mutants showed a significant proliferation defect over 96 h, suggesting that P. falciparum MS is not a vulnerable antimalarial target. Copyright 2024 Leela et al.
