Evolutionarily conserved amino acids in MHCII mediate bat influenza A virus entry into human cells

Abstract

AU The:viral Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly hemagglutinins of conventional influenza A viruses: (IAVs) bind to sialylated glycans on host cell surfaces for attachment and subsequent infection. In contrast, hemagglutinins of bat-derived IAVs target major histocompatibility complex class II (MHC-II) for cell entry. MHC-II proteins from various vertebrate species can facilitate infection with the bat IAV H18N11. Yet, it has been difficult to biochemically determine the H18:MHC-II binding. Here, we followed a different approach and generated MHC-II chimeras from the human leukocyte antigen DR (HLA-DR), which supports H18-mediated entry, and the nonclassical MHC-II molecule HLA-DM, which does not. In this context, viral entry was supported only by a chimera containing the HLA-DR ?1, ?2, and ?1 domains. Subsequent modeling of the H18:HLA-DR interaction identified the ?2 domain as central for this interaction. Further mutational analyses revealed highly conserved amino acids within loop 4 (N149) and ?sheet 6 (V190) of the ?2 domain as critical for virus entry. This suggests that conserved residues in the ?1, ?2, and ?1 domains of MHC-II mediate H18-binding and virus propagation. The conservation of MHC-II amino acids, which are critical for H18N11 binding, may explain the broad species specificity of this virus. Copyright: ? 2023 Olajide et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.