Enhanced high ?-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals
Author
Thumkasem N., On-mee T., Kongsinkaew C., Chittapun S., Pornpukdeewattana S., Ketudat-Cairns M., Thongprajukaew K., Antimanon S., Charoenrat T.
Affiliations
Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani12120, Thailand; Chakri Naruebodindra Medical Institute (CNMI), Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan, Bang Phli, 10540, Thailand
Type
Article
Source Title
Toxicology Reports
ISSN
22147500
Year
2024
Volume
12
Page
397-403
Open Access
All Open Access, Gold
Publisher
Elsevier Inc.
DOI
10.1016/j.toxrep.2024.03.011
Abstract
Investigating fine particulate matter (PM2.5) toxicity is crucial for health risk assessment and pollution control. This study explores the developmental toxicity of two PM2.5 sources: standard reference material 2786 (NIST, USA) and PM2.5 from Chakri Naruebodindra Medical Institute (CNMI, Thailand) located in the Bangkok Metropolitan area. Zebrafish embryos exposed to these samples exhibited embryonic mortality, with 50% lethal concentration (LC50) values of 1476 ?g/mL for standard PM2.5 and 512 ?g/mL for CNMI PM2.5. Morphological analysis revealed malformations, including pericardial and yolk sac edema, and blood clotting in both groups. Gene expression analysis highlighted source-specific effects. Standard PM2.5 downregulated sod1 and cat while upregulating gstp2. Inflammatory genes tnf-? and il-1b were upregulated, and nfkbi-?a was downregulated. Apoptosis-related genes bax, bcl-2, and casp3a were downregulated. CNMI PM2.5 consistently downregulated all examined genes. These findings underscore PM2.5 source variabilitys significance in biological system impact assessment, providing insights into pollutant-gene expression interactions. The study emphasizes the need for source-specific risk assessment and interventions to address PM2.5 exposures health impacts effectively. ? 2024 The Authors
