• Enhanced and asymmetric signatures of hybridization at climatic margins: Evidence from closely related dioecious fig species

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Metadata

Document Title

Enhanced and asymmetric signatures of hybridization at climatic margins: Evidence from closely related dioecious fig species

Author

Huang J.-F., Darwell C.T., Peng Y.-Q.

Affiliations

Faculty of Science, Burapha University, Chonburi, 20131, Thailand; Marine Biotechnology Research Unit, Faculty of Marine Technology, Burapha University, Chanthaburi Campus, Chanthaburi, 22170, Thailand; Marine Biotechnology Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand; Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Aquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Faculty of Science and Arts, Burapha University, Chanthaburi Campus, Chanthaburi, 22170, Thailand

Type

Article

Source Title

Aquaculture and Fisheries

ISSN

20961758

Year

2024

Open Access

All Open Access, Gold

Publisher

KeAi Communications Co.

DOI

10.1016/j.aaf.2024.04.011

Abstract

Genes exhibiting differential expression patterns between large-sized (average body weight 17.57 ? 1.8 g and 40.97 ? 4.0 g for 3- and 5-month-old juveniles in a total of 342 and 356 samples, respectively) and small-sized (average body weight 10.01 ? 0.76 g and 22.86 ? 2.51 g, respectively) juvenile giant tiger shrimps (Penaeus monodon) were isolated by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP). In total, 368 primer combinations were screened against the first-strand cDNA of the different groups of 3- and 5-month-old P. monodon. Over 146 differentially expressed or size-specific markers were derived using 59 selective primer combinations. A total of 70 candidate cDNA-AFLP markers—19 size-specific and 51 showing differential expression—were further cloned, sequenced, and blasted against the GenBank database. Twenty-five markers were found to comprise newly identified sequences (E-values >10?4). Quantitative real-time PCR and Single-strand conformational polymorphism (SSCP) analyses of the products generated with the SCAR/cDNA-AFLP primers were further undertaken. Interestingly, the relative expression levels of the products of primers E+34M+314-550-PDI, E+35M+310-350-Unk, E+29/M+211-350-CHK, E+34/M+31-380-phosphotysosyl, and E+38/M+315-800-fucosidase were significantly upregulated in the hepatopancreas of large-sized juvenile shrimp compared with that is small-sized animals. In contrast, the relative expression levels of the products generated by E+31M+38-480-InositolOxy and E+29/M+211-330-vitelline membrane were significantly upregulated in small-sized P. monodon. The consistency of our results was further assessed in 1-month-old aquaculture-raised P. monodon. Single nucleotide polymorphisms were identified using SSCP at different positions in the sequences of fragments of the different groups generated with the primers E+34M+314-550-PDI and E+35M+310-350-Unk. Our results indicated that cDNA-AFLP has the potential for use in the isolation of functionally important transcripts in P. monodon. ? 2024 Shanghai Ocean University.

License

CC BY-NC-ND

Rights

Shanghai Ocean University

Publication Source

Scopus

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