• Empowering an Acute Kidney Injury 3D Graphene-Based Sensor Using Extreme Learning Machine

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06/12/2024 by   

Metadata

Document Title

Empowering an Acute Kidney Injury 3D Graphene-Based Sensor Using Extreme Learning Machine

Author

Sittihakote N., Danvirutai P., Anutrakulchai S., Tuantranont A., Srichan C.

Affiliations

Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya464-8603, Japan; Department of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama, 226-8501, Japan; Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya464-8603, Japan; Research Institute for Electronic Science (RIES), Hokkaido University, N21W10, Kita, Hokkaido, Sapporo, 001-0021, Japan; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; National Nanotechnology Center (NANOTEC), NSTDA, Pathum Thani, 12120, Thailand; College of Materials Innovation and Technology, King Mongkut's Institute of Technology Ladkrabang, Chalongkrung Rd., Ladkrabang, Bangkok, 10520, Thailand; Institute of Quantum Life Science, National Institutes for Quantum Science and Technology (QST), Anagawa 4-9-1, Inage-ku, Chiba263-8555, Japan

Type

Article

Source Title

Biosensors and Bioelectronics

ISSN

9565663

Year

2024

Volume

254

Open Access

All Open Access, Hybrid Gold

Publisher

Elsevier Ltd

DOI

10.1016/j.bios.2024.116218

Abstract

Biodetection for non-invasive diagnostics of fluids, especially urine, remains a challenge to scientists due to low target concentrations. And biological complexes of the detection target may contain contaminants that also interfere with any assay. Dengue non-structural 1 protein (Dengue NS1) is an important biomarker for dengue hemorrhagic fever and dengue shock syndrome. Here, we developed an Au-decorated nanowire platform and applied it with a sandwich fluorophore-linked immunosorbent well plate assay (FLISA) to detect Dengue NS1 in urine. For the platform, we fabricated zinc oxide (ZnO) nanowires to provide a high surface area and then coated them with gold nanoparticles (ZnO/Au nanowires) to simply modify the Dengue NS1 antibody and enhance the fluorescence intensity. Our platform employs a sandwich FLISA that exhibits high sensitivity, specifically detecting Dengue NS1 with a limit of detection (LOD) of 1.35 pg/mL. This LOD was 4500-fold lower than the LOD of a commercially available kit for Dengue NS1 enzyme-linked immunosorbent assay. We believe that our ZnO/Au nanowire platform has the potential to revolutionize the field of non-invasive diagnostics for dengue. ? 2024 The Authors

License

CC BY-NC-ND

Rights

Authors

Publication Source

WoS

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