• Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

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Metadata

Document Title

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Author

Chaipanya C., Telebanco-Yanoria M.J., Quime B., Longya A., Korinsak S., Korinsak S., Toojinda T., Vanavichit A., Jantasuriyarat C., Zhou B.

Name from Authors Collection

Chaipanya C.

Scopus Author ID

57194048947

ORCID ID

NULL

|

Telebanco-Yanoria M.J.

Scopus Author ID

16043753800

ORCID ID

NULL

|

Quime B.

Scopus Author ID

57194204724

ORCID ID

NULL

|

Longya A.

Scopus Author ID

57193824051

ORCID ID

NULL

|

Korinsak S.

Scopus Author ID

57194208744

ORCID ID

NULL

|

Korinsak S.

Scopus Author ID

37117092000

ORCID ID

0000-0001-8021-6212

|

Theerayut Toojinda

Scopus Author ID

6602224071

ORCID ID

0000-0003-2828-5506

|

Vanavichit A.

Scopus Author ID

6603416422

ORCID ID

NULL

|

Jantasuriyarat C.

Scopus Author ID

6507379922

ORCID ID

NULL

|

Zhou B.

Scopus Author ID

55628584031

ORCID ID

NULL

Affiliations

Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand; Genetics and Biotechnology Division, International Rice Research Institute, Los Baños, Laguna 4031, Philippines; Rice Gene Discovery Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Plant Biotechnology Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Rice Science Center, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand; Agronomy Department Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand; Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASNAR, NRU-KU), Chatuchak, Bangkok 10900, Thailand

Type

Article

Source Title

Rice

ISSN

19398425

Year

2017

Volume

10

Issue

1

Open Access

Gold, Green

Publisher

Springer New York LLC

DOI

10.1186/s12284-017-0159-0

Abstract

Background: Rice (Oryza sativa) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 (QTL1) and 11 (QTL11). Results: Monogenic lines of QTL1 (QTL1-C) and QTL11 (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S (Pish) and IRBL7-M (Pi7), respectively. Moreover, sequence analysis revealed that Pish and Pi7 were embedded within the QTL1 and QTL11 delimited genomic intervals, respectively. This study thus concluded that QTL1 and QTL11 could encode alleles of Pish and Pi7, designated as Pish-J and Pi7-J, respectively. To validate this hypothesis, the genomic regions of Pish-J and Pi7-J were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C. Conclusion: JHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes, Pish-J and Pi7-J, in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of Pish and Pi7 can provide a practical scheme for breeding durable resistance in rice against rice blast disease. © 2017, The Author(s).

Knowledge Taxonomy Level 1

Biological Sciences

Knowledge Taxonomy Level 3

Plant biotechnology

Funding Sponsor

Lee Foundation; National Science and Technology Development Agency; Kasetsart University Research and Development Institute; National Center for Genetic Engineering and Biotechnology

License

CC BY

Rights

Author

Publication Source

Scopus

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