Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms
Development of Immersion and Oral Bivalent Nanovaccines for Streptococcosis and Columnaris Disease Prevention in Fry and Fingerling Asian Seabass (Lates calcarifer) Nursery Farms
Author
Meachasompop P. Bunnoy A. Keaswejjareansuk W. Dechbumroong P. Namdee K. Srisapoome P.
Affiliations
Laboratory of Aquatic Animal Health Management Department of Aquaculture Faculty of Fisheries Kasetsart University 50 Paholayothin Rd Ladyao Chatuchak Bangkok 10900 Thailand; Center of Excellence in Aquatic Animal Health Management Faculty of Fisheries Kasetsart University 50 Paholayothin Rd Ladyao Chatuchak Bangkok 10900 Thailand; National Nanotechnology Center (NANOTEC) National Science and Technology Development Agency (NSTDA) Science Park Pathumthani 12120 Thailand
Type
Article
Source Title
Vaccines
ISSN
2076393X
Year
2024
Volume
12
Issue
1
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/vaccines12010017
Abstract
In the present study chitosan-based bivalent nanovaccines of S. iniae and F. covae were administered by immersion vaccination at 30 and 40 days after hatching (DAH) and the third vaccination was orally administered by feeding at 50 DAH. ELISA revealed that the levels of total IgM and specific IgM to S. iniae and F. covae were significantly elevated in all vaccinated groups at 10 20 and 30 days after vaccination (DAV). A qRT-PCR analysis of immune-related genes revealed significantly higher IgT expression in the vaccinated groups compared to the control group as revealed by 44�0-fold changes in the vaccinated groups compared to the control (p < 0.001) at every tested time point after vaccination. All vaccinated groups expressed IgM MHCII? and TCR? at significantly higher levels than the control group at 10 and/or 20 DAV (p < 0.05). In the S. iniae challenge tests the survival of vaccinated groups ranged from 62.15 ? 2.11 to 75.70 ? 3.36% which significantly differed from that of the control group (44.44 ? 1.92%). Similarly all vaccinated groups showed higher survival rates of 68.89 ? 3.85 to 77.78 ? 5.09% during F. covae challenge than the control groups (50.00 ? 3.33%) (p < 0.05). ? 2023 by the authors.
