King Mongkuts University of Technology Thonburi; Universitas Islam Indonesia; Mahidol University; King Mongkuts University of Technology Thonburi; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Phramongkutklao College of Medicine; Phramongkutklao Hospital
Type
Article
Source Title
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Year
2021
Volume
107
Page
271-277
Open Access
gold
Publisher
ELSEVIER SCI LTD
DOI
10.1016/j.ijid.2021.05.007
Format
PDF
Abstract
Objectives: This study aimed to evaluate a microfluidic paper-based analytical device (DEN-NS1-PAD) based on a rapid NS1 antigen test for diagnosing dengue at the point of care. Methods: 219 serum samples from suspected dengue cases were tested with the developed DEN-NS1PAD and commercial RDT by SD BIOLINE. The results were compared with the nested-PCR results. Results: The limit of detection of DEN-NS1-PAD was 0.78 ng mL(1). It showed 88.89% sensitivity, 86.67% specificity, and a substantial agreement correlation (k = 0.7522) compared with nested-PCR. In contrast, SD BIOLINE for NS1 (SD-NS1) detection showed 87.88% sensitivity, 90.00% specificity, and had a substantial agreement correlation with nested-PCR (k = 0.7788). Conclusions: DEN-NS1-PAD is a valuable tool for diagnosing DENV infections, especially for diagnosed patients with early acute phase samples with high viral load. DEN-NS1-PAD has better sensitivity than SD-NS1 but less specificity. (C) 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
Universitas Islam Indonesia, Indonesia; King Mongkut's University of Technology Thonburi, Thailand through the KMUTT 55th Anniversary Commemorative Fund
