• Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

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Document Title

Characterization of a novel yeast phase-specific antigen expressed during in vitro thermal phase transition of Talaromyces marneffei

Author

Pruksaphon K.,Ching M.M.N.,Nosanchuk J.D.,Kaltsas A.,Ratanabanangkoon K.,Roytrakul S.,Martinez L.R.,Youngchim S.

Name from Authors Collection

Pruksaphon K.

Scopus Author ID

57188553670

ORCID ID

NULL

|

Ching M.M.N.

Scopus Author ID

57217151009

ORCID ID

NULL

|

Nosanchuk J.D.

Scopus Author ID

7005300850

ORCID ID

NULL

|

Kaltsas A.

Scopus Author ID

24447954100

ORCID ID

NULL

|

Ratanabanangkoon K.

Scopus Author ID

7004082532

ORCID ID

NULL

|

Sittiruk Roytrakul

Scopus Author ID

6508382189

ORCID ID

0000-0003-3696-8390

|

Martinez L.R.

Scopus Author ID

7201890318

ORCID ID

NULL

|

Youngchim S.

Scopus Author ID

6602766297

ORCID ID

NULL

Affiliations

Graduate Program in Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns School of Medicine, University of Hawaii at Mānoa, Honolulu, HI 96813, United States; Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, United States; Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, United States; Infectious Disease Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, United States; Department of Medicine, Joan and Sanford Weill Cornell Medical College, Cornell University, New York, NY 10065, United States; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand; Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, United States; Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

Type

Article

Source Title

Scientific Reports

ISSN

20452322

Year

2020

Volume

10

Issue

1

Open Access

All Open Access, Gold

Publisher

Nature Research

DOI

10.1038/s41598-020-78178-5

Abstract

Talaromyces marneffei is a dimorphic fungus that has emerged as an opportunistic pathogen particularly in individuals with HIV/AIDS. Since its dimorphism has been associated with its virulence, the transition from mold to yeast-like cells might be important for fungal pathogenesis, including its survival inside of phagocytic host cells. We investigated the expression of yeast antigen of T.marneffei using a yeast-specific monoclonal antibody (MAb) 4D1 during phase transition. We found that MAb 4D1 recognizes and binds to antigenic epitopes on the surface of yeast cells. Antibody to antigenic determinant binding was associated with time of exposure, mold to yeast conversion, and mammalian temperature. We also demonstrated that MAb 4D1 binds to and recognizes conidia to yeast cells’ transition inside of a human monocyte-like THP-1 cells line. Our studies are important because we demonstrated that MAb 4D1 can be used as a tool to study T.marneffei virulence, furthering the understanding of the therapeutic potential of passive immunity in this fungal pathogenesis. © 2020, The Author(s).

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Knowledge Taxonomy Level 2

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Knowledge Taxonomy Level 3

N/A

License

CC BY

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Publication Source

Scopus

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