• Characterization and utilization of cacao shell powder as a biofiller in natural rubber composite

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06/12/2024 by   

Metadata

Document Title

Characterization and utilization of cacao shell powder as a biofiller in natural rubber composite

Author

Wetchakama P., Sriwongruang D., Sae-Oui P., Siriwong C.

Affiliations

Department of Biology, Faculty of Science, Mahasarakham University, Mahasarakham, 44150, Thailand; Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Khonkaenwittayayon School, Khon Kaen, 40000, Thailand; Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani12120, Thailand; Department of Biotechnology, Faculty of Agricultural Technology, Kalasin University, Kalasin46000, Thailand; Department of Integrated Science, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Pharmaceutical Technology, Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

Type

Article

Source Title

Heliyon

ISSN

24058440

Year

2024

Volume

10

Issue

11

Open Access

All Open Access, Gold

Publisher

Elsevier Ltd

DOI

10.1016/j.heliyon.2024.e32468

Abstract

A simple method to generate antibacterial peptides by alkaline hydrolysis of hen egg whites is reported. The method reproducibly generates short peptides with molecular weight of less than 14.4 kDa that exhibit low to no cytotoxicity on RAW 264.7 macrophage cells, but do inhibit the bacterial growth of Cutibacterium acnes (C. acnes), Staphylococcus aureus (S. aureus) and antibiotic-resistant S. aureus (MRSA), while also reducing nitric oxide production from heat-killed C. acnes-treated RAW 264.7 cells. Peptidomics revealed at least thirty peptides within the complex mixture, of which eight were evaluated individually. Three peptides (PK8, EE9 and RP8) were potent anti-inflammation and antibacterial agents, but notably the complex egg white hydrolysate (EWH) was more effective than the individual peptides. Electron microscopy suggests the antibacterial mechanism of both the hydrolysate and the selected peptides is through disruption of the cell membrane of C. acnes. These findings suggest that EWH and EWH-derived peptides are promising candidates for infection and inflammation treatment, particularly in managing acne and combating antibiotic-resistant bacteria like MRSA. ? 2024

License

CC BY-NC-ND

Rights

Authors

Publication Source

WoS

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