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Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement
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Metadata
Document Title
Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement
Author
Harnpicharnchai P, Sornlake W, Tang K, Eurwilaichitr L, Tanapongpipat S
Name from Authors Collection
Scopus Author ID
6602764100
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097
Year
2010
Volume
302
Issue
2-3
Open Access
Bronze
Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI
10.1111/j.1574-6968.2009.01811.x
Format
Abstract
Cell-surface expression of phytase allows the enzyme to be expressed and anchored on the cell surface of Pichia pastoris. This avoids tedious downstream processes such as purification and separation involved with extracellular expression. In addition, yeast cells with anchored proteins can be used as a whole-cell biocatalyst with high value added. In this work, the phytase was expressed on the cell surface of P. pastoris with a glycosylphosphatidylinositol anchoring system. The recombinant phytase was shown to be located at the cell surface. The cell-surface phytase exhibited high activity with an optimal temperature at 50-55 degrees C and two optimal pH peaks of 3 and 5.5. The surface-displayed phytase also exhibited similar pH stability and pepsin resistance to the native and secreted phytase. In vitro digestibility test showed that P. pastoris containing cell-surface phytase released phosphorus from feedstuff at a level similar to secreted phytase. Yeast cells expressing phytase also provide additional nutrients, especially biotin and niacin. Thus, P. pastoris with phytase displayed on its surface has a great potential as a whole-cell supplement to animal feed.
Keyword
agglutinin | cell-surface enzyme | feed supplement | Phytase | Pichia pastoris
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Center for Genetic Engineering and Biotechnology (BIOTEC); National Science and Technology Development Agency (NSTDA), Thailand [BT-B-02-LG-BI-5102]
License
Copyright
Rights
Federation of European Microbiological Societies
Publication Source
WOS