• Bromelain Immobilized onto Clay-Carboxymethylcellulose Composites for Improving Nutritive Value of Soybean Meal

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Metadata

Document Title

Bromelain Immobilized onto Clay-Carboxymethylcellulose Composites for Improving Nutritive Value of Soybean Meal

Author

Pimcharoen K., Opaprakasit P., Yingchutrakul Y., Simanon N., Butkinaree C., Yuttayong D., Hompa R., Vayachuta L., Prompinit P.

Affiliations

School of Integrated Science and Innovation, Sirindhorn International Institute of Technology (SIIT), Thammasat University, Pathum Thani, 12121, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (NSTDA), Pathum Thani, Khlong Luang, 12120, Thailand; Aquatic Animal Feed Research and Development Division, Department of Fisheries, Ministry of Agriculture and Cooperatives, Bangkok, 10900, Thailand; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Khlong Luang, 12120, Thailand

Source Title

ACS Applied Bio Materials

ISSN

25766422

Year

2024

Open Access

All Open Access, Hybrid Gold

Publisher

American Chemical Society

DOI

10.1021/acsabm.4c00392

Abstract

Improvement of nutritional value and reduction of antinutritional factors (ANFs) of soybean meal (SBM) for animal feed applications could be achieved by using bromelain immobilized onto bentonite (Bt)-carboxymethylcellulose (CMC) composites. The composite with mass ratio between CMC to calcium ion (Ca2+) at 1:20 provided the highest enzyme activity, immobilization yield higher than 95%, with superior thermal and storage stabilities. Performance of the immobilized bromelain for soybean protein hydrolysis was further studied. The results showed that at 60 °C, the immobilized bromelain exhibited the highest efficiency in enzymatic hydrolysis to release free alpha amino nitrogen (FAN) as a product with high selectivity and to effectively reduce SBM allergenic proteins within 30 min. In conclusion, immobilization of bromelain onto Bt-CMC composites leads to stability enhancement of the enzyme, enabling effective improvement in SBM quality in a short treatment time and showing great potential for application in animal feed industries. © 2024 The Authors. Published by American Chemical Society.

Knowledge Taxonomy Level 1

Chemical Science

Knowledge Taxonomy Level 2

Nanoabsorbant | nanofilter and nanocatalyst

Knowledge Taxonomy Level 3

Nanoabsorbant | nanofilter and nanocatalyst

License

CC BY

Rights

Authors

Publication Source

Scopus

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