-
Bioinformatic Prediction of Novel Signaling Pathways of Apoptosis-inducing Factor, Mitochondrion-associated 3 (AIFM3) and Their Roles in Metastasis of Cholangiocarcinoma Cells
- Back
Metadata
Document Title
Bioinformatic Prediction of Novel Signaling Pathways of Apoptosis-inducing Factor, Mitochondrion-associated 3 (AIFM3) and Their Roles in Metastasis of Cholangiocarcinoma Cells
Author
Chua-On D, Proungvitaya T, Techasen A, Limpaiboon T, Roytrakul S, Tummanatsakun D, Araki N, Proungvitaya S
Name from Authors Collection
Affiliations
Khon Kaen University; Khon Kaen University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Kumamoto University
Type
Article
Source Title
CANCER GENOMICS & PROTEOMICS
ISSN
1109-6535
Year
2022
Volume
19
Issue
1
Page
35-49
Open Access
Green Published, gold
Publisher
INT INST ANTICANCER RESEARCH
DOI
10.21873/cgp.20302
Format
Abstract
Background/Aim: We previously demonstrated that a mitochondrial protein, apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is over-expressed in cholangiocarcinoma (CCA) and its serum levels can be a prognostic biomarker for CCA. To elucidate the functional roles of AIFM3 in CCA progression, we aimed to determine the signaling pathways of AIFM3 in CCA. Materials and Methods: AIFM3 gene in CCA cells was silenced and AIFM3related proteins were identified using mass spectrometry and bioinformatics tools. The relationships between AIFM3 and 441 related proteins were explored. To validate the functions of AIFM3, transwell migration/invasion assays were used. Results: Bioinformatic analyses predicted that AIFM3 interacts with formin-like protein 3 (FMNL3) and is involved in tumor cell motilities. Online database analysis revealed higher AIFM3 mRNA expression levels in CCA, particularly with lymph node metastasis. After AIFM3 gene silencing, CCA cell migration/invasion was significantly decreased (p<0.001). Furthermore, AIFM3 expression levels were significantly associated with lymph node metastasis (p=0.0009) and shorter survival time (p=0.020). Conclusion: The AIFM3 signaling pathway is mediated via FMNL3 and involved in metastasis, suggesting that AIFM3 might be a molecular target to prevent CCA metastasis.
Keyword
AIFM3 | Bioinformatics | Cholangiocarcinoma | Mass spectrometry | Metastasis | Signaling pathway
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program - Khon KaenUniversity [PHD/0065/2559, 61003505]; Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
License
Copyright
Rights
International Institute of Anticancer Research (Dr. George J. Delinasios)
Publication Source
WOS