• Bimetallic nanoparticles with sulfated galactan eliminate Vibrio parahaemolyticus in shrimp Penaeus vannamei

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06/12/2024 by   

Metadata

Document Title

Bimetallic nanoparticles with sulfated galactan eliminate Vibrio parahaemolyticus in shrimp Penaeus vannamei

Author

Kamble M.T., Soowannayan C., Chaicherd S., Medhe S.V., Rudtanatip T., Pissuwan D., Wongprasert K.

Affiliations

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Toxicology Program, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Materials Science and Engineering Program, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Center of Excellence in Wildlife, Exotic, and Aquatic Animal Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand; Department of Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand; Nanobiotechnology and Nanobiomaterials Research (N-BMR) Laboratory, School of Materials Science and Innovation, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

Source Title

Fish and Shellfish Immunology

ISSN

10504648

Year

2024

Volume

151

Open Access

All Open Access, Hybrid Gold

Publisher

Academic Press

DOI

10.1016/j.fsi.2024.109753

Abstract

Bimetallic (Au/Ag) nanoparticles (BNPs) have shown enhanced antibacterial activity compared to their monometallic counterparts. Sulfated galactans (SG) are a naturally occurring polymer commonly found in red seaweed Gracilaria fisheri. They are biocompatible and biodegradable and environmentally friendly. In this study, we utilized SG in combination with BNPs to develop composite materials that potentially enhance antibacterial activity against shrimp pathogens Vibrio parahaemolyticus and Vibrio harveyi, compared to BNPs or SG alone. BNPs were coated with sulfated galactan (SGBNPs) and characterized using UV–vis spectroscopy, Fourier transform infrared (FTIR) spectroscopy, zeta potential, and transmission electron microscopy (TEM). UV–vis spectroscopy analysis revealed that the surface plasmon peaks of BNPs and SGBNPs appeared at 530 nm and 532 nm, respectively. Zeta potential measurements showed that SGBNPs had a negative charge of −32.4 mV, while the BNPs solution had a positive charge of 38.7 mV. TEM images demonstrated the spherical morphology of both BNPs and SGBNPs with narrow size distributions (3–10 nm). Analysis of the FTIR spectra indicated that SG maintained its backbone structure in SGBNPs, but some functional groups were altered. Notably, SGBNPs showed superior antimicrobial and antibiofilm activities against V. parahaemolyticus and V. harveyi compared to SG and BNPs. Furthermore, treatment with SGBNPs significantly down-regulated the expression of virulence-related genes (toxR, cpsQ, and mfpA) for V. parahaemolyticus 3HP compared to the respective control, bacteria treated with BNPs or SG. Diets supplemented with SGBNPs, BNPs, or SG showed no detrimental impact on the growth of shrimp Penaeus vannamei. Shrimp fed with SGBNPs-supplemented feed showed significantly higher survival rates than those fed with BNPs-supplemented feed when infected with 3HP after being on the supplemented feed for seven days and a subsequent number of fifteen days. These findings collectively demonstrate the benefit of using SG capped Au–Ag BNPs as an antibacterial agent for the prevention and control of Vibrio sp. Infection in shrimp while reducing the risk of environmental contamination. © 2024 The Authors

License

CC BY-NC-ND

Rights

Authors

Publication Source

Scopus

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