Khon Kaen University; National Institute of Infectious Diseases (NIID); Chiang Mai University; Khon Kaen University; Khon Kaen University; National Institute of Infectious Diseases (NIID); Khon Kaen University; Khon Kaen University; Chiang Mai University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Khon Kaen University; Khon Kaen University; Khon Kaen University
Type
Article
Source Title
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
ISSN
0002-9637
Year
2013
Volume
89
Issue
1
Page
63-67
Open Access
Green Published
Publisher
AMER SOC TROP MED & HYGIENE
DOI
10.4269/ajtmh.12-0617
Format
PDF
Abstract
Matrix metalloproteinase (MMPs) is the extracellular zinc-dependent endopeptidase and is secreted for degrading extracellular matrix molecules of host tissues. A cDNA encoding MMP-like protein of Gnathostoma spinigerum larvae was amplified by reverse transcription-polymerase chain reaction, and was cloned into a prokaryotic expression vector, and expressed in Escherichia coli. Total immunoglobulin G class (total IgG) antibody responses to the recombinant MMP-like protein were analyzed by immunoblot diagnosis of human gnathostomiasis. Serum samples from proven and clinically suspected cases of gnathostomiasis, other parasitic diseases patients, and from healthy volunteers were tested. The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85.4% and 100%, respectively. Recombinant MMP-like protein can be used as a diagnostic antigen and potentially replace native parasite antigens to develop a gnathostomiasis diagnostic kit.
Funding Sponsor
Office of the Higher Education Commission, Thailand; Higher Education Research Promotion and National Research University Project of Thailand; Faculty of Medicine, Khon Kaen University; CHE PhD scholarship; TRF Senior Research Scholar Grant, Thailand Research Fund [RTA5580004]; Association for Preventive Medicine of Japan
