Mahidol University; Mahidol Oxford Tropical Medicine Research Unit (MORU); Mahidol University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University
Type
Article
Source Title
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
ISSN
0035-9203
Year
2012
Volume
106
Issue
10
Page
632-635
Open Access
Green Published
Publisher
ELSEVIER SCIENCE INC
DOI
10.1016/j.trstmh.2012.05.004
Format
PDF
Abstract
The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r=0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. (C) 2012 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
