Abstract
A novel actinobacterial strain, designated C12CA1(T), was isolated from forest soil in the conservation area of Chulabhorn dam, Thailand, and its taxonomic position was determined by using a polyphasic approach. Strain C12CA1(T) contained meso-2,6 diaminopimelic acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars of the whole-cell hydrolysate. On the basis of morphological and chemotaxonomic characteristics, strain C12CA1(T) was classified in the genus Amycolatopsis. It contained MK-9(H-4) as the predominant menaquinone, C-16:0, iso-C-15:0 and iso-C-16:0 as the major cellular fatty acids, and several phospholipids consisting of diphosphotidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, hydroxyphosphatidylethanolamine, phosphatidylinositol mannosides and an unidentified glucosamine-containing phospholipid. Based on 16S rRNA gene sequence and phylogenetic analyses, strain C12CA1(T) was closely related to Amycolatopsis vancoresmycina DSM 44592(T) (98.96 %) and Amycolatopsis pretoriensis JCM 12673(T) (98.82 %). The strain exhibited low DNA-DNA relatedness values with A. vancoresmycina DSM 44592(T) (6.9 +/- 0.2-11.6 +/- 1.9 %) and A. pretoriensis JCM 12673(T) (8.8 +/- 0.3-9.2 +/- 1.8 %). The DNA G+C content of strain C12CA1(T) was 69.8 mol%. Based on the results of polyphasic characterization, strain C12CA1(T) represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis silviterrae sp. nov. is proposed. The type strain is C12CA1(T) (=TBRC 1456(T)=NBRC 111116(T)).
