National Science & Technology Development Agency - Thailand; National Nanotechnology Center (NANOTEC); Mahidol University; Kasetsart University; Kasetsart University; Thammasat University; University of Strathclyde
Type
Article
Source Title
METHODSX
Year
2020
Volume
7
Open Access
Green Published, gold, Green Accepted
Publisher
ELSEVIER
DOI
10.1016/j.mex.2020.101114
Format
PDF
Abstract
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 mu g.mL(-1) and the detection range is 0.1- 600 mu g.mL(-1), which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a highthroughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample The limit of detection of our developed platform is 0.05 mu g.mL(-1) The detection range of our aptasensor is 0.1-600 mu g.mL(-1) (C) 2020 The Authors. Published by Elsevier B.V.
