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Acetobacter sacchari sp. nov., for a plant growth-promoting acetic acid bacterium isolated in Vietnam
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Metadata
Document Title
Acetobacter sacchari sp. nov., for a plant growth-promoting acetic acid bacterium isolated in Vietnam
Author
Vu H.T.L.,Yukphan P.,Bui V.T.T.,Charoenyingcharoen P.,Malimas S.,Nguyen L.K.,Muramatsu Y.,Tanaka N.,Tanasupawat S.,Le B.T.,Nakagawa Y.,Yamada Y.
Name from Authors Collection
Affiliations
Department of Microbiology, Faculty of Biology and Biotechnology, University of Science, Vietnam National University-HCM City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Cau Giay District, Hanoi, Viet Nam; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Rungrueng-Fertilizer Co., Ltd, 207/5 Moo1, Nong Ya, Muang Kanchanaburi, Kanchanaburi, 71000, Thailand; NITE Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusa-Kamatari, Kisarazu, 292-0818, Japan; Department of Molecular Microbiology, NODAI Culture Collection Center, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan; Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand; Japan International Cooperation Agency (JICA Senior Overseas Volunteer), Shibuya-ku, Tokyo, 151-8558, Japan; Laboratory of Applied Microbiology (Professor Emeritus), Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Suruga-ku, Shizuoka, 422-8529, Japan
Type
Article
Source Title
Annals of Microbiology
ISSN
15904261
Year
2019
Volume
69
Issue
11
Page
1155-1163
Open Access
All Open Access, Bronze
Publisher
Springer Verlag
DOI
10.1007/s13213-019-01497-0
Abstract
Purpose: Two bacterial strains, designated as isolates VTH-Ai14T and VTH-Ai15, that have plant growth-promoting ability were isolated during the study on acetic acid bacteria diversity in Vietnam. The phylogenetic analysis based on 16S rRNA gene sequences showed that the two isolates were located closely to Acetobacter nitrogenifigens RG1T but formed an independent cluster. Methods: The phylogenetic analysis based on 16S rRNA gene and three housekeeping genes’ (dnaK, groEL, and rpoB) sequences were analyzed. The genomic DNA of the two isolates, VTH-Ai14T and VTH-Ai15, Acetobacter nitrogenifigens RG1T, the closest phylogenetic species, and Acetobacter aceti NBRC 14818T were hybridized and calculated the %similarity. Then, phenotypic and chemotaxonomic characteristics were determined for species’ description using the conventional method. Results: The 16S rRNA gene and concatenated of the three housekeeping genes phylogenetic analysis suggests that the two isolates were constituted in a species separated from Acetobacter nitrogenifigens, Acetobacter aceti, and Acetobacter sicerae. The two isolates VTH-Ai14T and VTH-Ai15 showed 99.65% and 98.65% similarity of 16S rRNA gene when compared with Acetobacter nitrogenifigens and Acetobacter aceti and they were so different from Acetobacter nitrogenifigens RG1T with 56.99 ± 3.6 and 68.15 ± 1.8% in DNA-DNA hybridization, when isolates VTH-Ai14T and VTH-Ai15 were respectively labeled. Moreover, the two isolates were phenotypically distinguished from Acetobacter nitrogenifigens in growth in the presence of 0.35% acetic acid (v/v), on nitrogen-free LGI medium and D-mannitol, and in no ability to solubilize phosphate. Conclusion: Therefore, the two isolates, VTH-Ai14T(= VTCC 910031T= BCC 67843T= TBRC 11175T= NRIC 0977T) and VTH-Ai15 (= VTCC 910032 = BCC 67844 = TBRC 11176 = NRIC 0978), can be assigned to an independent species within the genus Acetobacter, and the name of Acetobacter sacchari sp. nov. is proposed for the two isolates. © 2019, Università degli studi di Milano.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus