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A simplified positive-sense-RNA virus construction approach that enhances analysis throughput
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Metadata
Document Title
A simplified positive-sense-RNA virus construction approach that enhances analysis throughput
Author
Siridechadilok B.,Gomutsukhavadee M.,Sawaengpol T.,Sangiambut S.,Puttikhunt C.,Chin-inmanu K.,Suriyaphol P.,Malasit P.,Screaton G.,Mongkolsapaya J.
Name from Authors Collection
Affiliations
National Center For Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathumthani, Thailand; Bioinformatics and Data Management for Research Unit, Office of Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand; Dengue Hemorrhagic Fever Unit, Office of Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand; Molecular Immunology Unit, Hammersmith Campus, Department of Medicine, Imperial College London, London, United Kingdom
Type
Article
Source Title
Journal of Virology
ISSN
0022538X
Year
2013
Volume
87
Issue
23
Open Access
All Open Access, Hybrid Gold, Green
DOI
10.1128/JVI.02261-13
Abstract
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. © 2013, American Society for Microbiology.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus